A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria.
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- NAGASHIMA Koji
- Hokkaido Food Processing Research Center
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- SHIMIZU Takeshi
- Hokkaido Food Processing Research Center
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- TAKESHI Kouichi
- Hokkaido Institute of Public Health
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- KAWAKAMI Makoto
- Hokkaido Food Processing Research Center
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- YASOKAWA Daisuke
- Hokkaido Food Processing Research Center
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- NAKAGAWA Ryoji
- Hokkaido Food Processing Research Center
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- OKUMURA Yukihiro
- Hokkaido Food Processing Research Center
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抄録
We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 treatment. Using template DNA prepared by this method, it was possible to detect as few as one colony forming unit of some kinds of food-related bacteria using universal primers targeting the bacterial 16S ribosomal RNA gene or specific primers for bacterial genes under fixed thermocycling conditions.
収録刊行物
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- Food Science and Technology Research
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Food Science and Technology Research 6 (2), 115-118, 2000
公益社団法人 日本食品科学工学会
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詳細情報
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- CRID
- 1390282679432718976
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- NII論文ID
- 130000061938
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- NII書誌ID
- AA11320122
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- COI
- 1:CAS:528:DC%2BD3cXmsVSgsr0%3D
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- ISSN
- 18813984
- 13446606
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可