A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria.

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We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 treatment. Using template DNA prepared by this method, it was possible to detect as few as one colony forming unit of some kinds of food-related bacteria using universal primers targeting the bacterial 16S ribosomal RNA gene or specific primers for bacterial genes under fixed thermocycling conditions.

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詳細情報

  • CRID
    1390282679432718976
  • NII論文ID
    130000061938
  • NII書誌ID
    AA11320122
  • DOI
    10.3136/fstr.6.115
  • COI
    1:CAS:528:DC%2BD3cXmsVSgsr0%3D
  • ISSN
    18813984
    13446606
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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