Identification and molecular characterization of novel anther-specific genes in japonica rice, Oryza sativa L. by using cDNA microarray
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- Endo Makoto
- Faculty of Agriculture, Iwate University Graduate School of Life Sciences, Tohoku University
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- Tsuchiya Tohru
- Life Science Research Center, Mie University
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- Saito Hiroshi
- Faculty of Agriculture, Iwate University
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- Matsubara Hitoshi
- Faculty of Agriculture, Iwate University
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- Hakozaki Hirokazu
- Faculty of Agriculture, Iwate University
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- Masuko Hiromi
- Faculty of Agriculture, Iwate University
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- Kamada Motoshi
- Graduate School of Life Sciences, Tohoku University
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- Higashitani Atsushi
- Graduate School of Life Sciences, Tohoku University
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- Takahashi Hideyuki
- Graduate School of Life Sciences, Tohoku University
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- Fukuda Hiroo
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo Plant Science Center, The Institute of Physical and Chemical Research (RIKEN)
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- Demura Taku
- Plant Science Center, The Institute of Physical and Chemical Research (RIKEN)
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- Watanabe Masao
- Faculty of Agriculture, Iwate University
書誌事項
- タイトル別名
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- Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray
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抄録
The complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. To understand these molecular mechanisms, we performed cDNA microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. Microarray analysis of 4,304 cDNA clones revealed that the hybridization signal of 396 cDNA clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of leaves. Cluster analysis with the expression data showed that 259 cDNA clones (156 non redundant groups) were specifically or predominantly expressed in anther tissues and were regulated by developmental stage-specific manners in the anther tissues. These co-regulated genes would be impor-tant for development of functional anther tissues. Furthermore, we selected several clones for RNA in situ hybridization analysis. From these analyses, we found several novel genes that show temporal and spatial expression patterns during anther development in addition to anther-specific genes reported so far. These results indicate that the genes identified in this experiment are controlled by different programs and are specialized in their developmental and cell types.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 79 (4), 213-226, 2004
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詳細情報
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- CRID
- 1390282680450736256
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- NII論文ID
- 130000062132
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
- http://id.crossref.org/issn/13417568
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- NDL書誌ID
- 7120479
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- PubMed
- 15514441
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可