Isolation and characterization of sea aster salt-stress responsive cystein protease gene obtained by a hetero-probed macroarray

• TAKEDA Migiwa

  Graduate School of Science and Technology, Kobe University

• KUWATA Chikara

  Kazusa DNA Research Institute

• UNO Yuichi

  Faculty of Agriculture, Kobe University

• KANECHI Michio

  Graduate School of Science and Technology, Kobe University

• SHIBATA Daisuke

  Kazusa DNA Research Institute

• INAGAKI Noboru

  Graduate School of Science and Technology, Kobe University

書誌事項

タイトル別名

• Isolation and Characterization of Sea Aster Salt-Stress Responsive Cysteine Protease Gene Obtained by a Hetero-probed Macroarray

抄録

To isolate and analyze salt-stress inducible genes in a halophyte, sea aster (Aster tripolium L.), we screened 5760 Arabidopsis cDNA clones by macroarray procedure using ³³P-labeled cDNA targets synthesized from mRNAs isolated from NaCl treated and untreated sea aster seedlings. Seventeen Arabidopsis cDNAs were hetero-hybridized to NaCl inducible sea aster genes. These cDNAs were used as probes to isolate cDNA homologs from a sea aster cDNA library. One of the obtained cDNAs shared 71% amino acids identity with Arabidopsis cysteine protease (AtCysP) and named SaCysP (sea aster CysP). Northern blot analysis revealed that mRNAs corresponding to both SaCysP and AtCysP were induced by salt and osmotic stress in leaves. On the other hand, SASR21 mRNA encoding another CysP in sea aster was irresponsive to these stress in leaves but respond in roots. SaCysP and SASR21 genes may have a tissue-specific function in stress response by modulating their expression levels.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 21 (2), 127-133, 2004

  日本植物バイオテクノロジー学会

キーワード

• Arabidopsis
• Cysteine protease
• Gene expression
• Macroarray
• Mannitol
• NaCl stress
• Sea aster (Aster tripolium L.)

詳細情報

• CRID: 1390282679305301248
• NII論文ID: 130000064310
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.21.127
• COI: 1:CAS:528:DC%2BD2cXlvVGrtbc%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 6960246
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6960246
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可