Detection of specific antibodies against infectious hematopoietic necrosis virus (IHNV) from rainbow trout sera by ELISA using two novirhabdoviruses

• Kim Wi-Sik

  Faculty of Fisheries Sciences, Hokkaido University

• Mochizuki Mamiko

  Fuji Trout Hatchery, Shizuoka Prefectural Research Institute of Fishery

• Nishizawa Toyohiko

  Faculty of Fisheries Sciences, Hokkaido University

• Yoshimizu Mamoru

  Faculty of Fisheries Sciences, Hokkaido University

書誌事項

タイトル別名

• Detection of Specific Antibodies against Infectious Hematopoietic Necrosis Virus from Rainbow Trout Sera by ELISA using Two Novirhabdoviruses

抄録

An enzyme-linked immunosorbent assay (ELISA) with viral culture fluids as capture antigens was performed to detect specific antibodies against infectious hematopoietic necrosis virus (IHNV) from rainbow trout Oncorhynchus mykiss sera. When using IHNV antigen (IHNV-Ag), ELISA OD values for IHN-survived (IHN-Surv) rainbow trout sera were relatively higher than those for specific pathogen free (SPF) fish sera. However, some of the SPF sera were diagnosed as positive due to high OD values (> 0.2). To clarify reasons for these high OD values, each of five IHN-Surv and SPF sera was subjected to ELISA with viral hemorrhagic septicemia virus (VHSV) and hirame rhabdovirus (HIRRV) antigens (VHSV-Ag and HIRRV-Ag). Some of the IHN-Surv and SPF sera showed high OD values in both VHSV-Ag and HIRRV-Ag plates even though there was no possibility that those sera contained antibodies against VHSV or HIRRV antigen. These results suggest that some of the sera contained antibodies against impurities in viral culture fluids such as FBS and cell debris, and caused the false-positive reactions. The corrected OD values, subtracted the OD values in VHSV-Ag plates from those in IHNV-Ag plates, were all > 0.2, in SPF sera (n = 148) while those in IHN-Surv sera (n = 238) were randomly distributed from 0 to 0.7. It was considered that the corrected OD values may represent true values recognized by IHNV-specific antibodies.

収録刊行物

• 魚病研究

  魚病研究 43 (3), 112-116, 2008

  日本魚病学会

キーワード

• antibody detection, ELISA
• <i>Oncorhynchus mykiss</i>
• IHNV
• background

詳細情報

• CRID: 1390282681437365504
• NII論文ID: 10024481030
• NII論文ID: 130000065679
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.43.112
• ISSN: 18817335
• ISSN: 0388788X
• HANDLE: 2115/39126
• NDL書誌ID: 9655468
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9655468
• 本文言語コード: en
• データソース種別:
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