Isolation and Characterization of a Novel Acid Proteinase, Tropiase from Candida tropicalis IFO 0589

• Okumura Yoshiyuki

  Department of Quality Control, Mathuurayakugyo Co., Ltd. Department of Microbiology, Faculty of Pharmacy, Meijo University

• Inoue Naomasa

  Scientific Affairs & Sales Promotion Group, Asahi Kasei Co., Ltd.

• Nikai Toshiaki

  Department of Microbiology, Faculty of Pharmacy, Meijo University

抄録

A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of pH 5.1, optimum pH range of 7 to 9 and possessed 208 amino acid residues. The enzyme hydrolyzed casein, fibrinogen, keratin and collagen. The purified tropiase demonstrated hemorrhagic and capillary permeability-increasing activities. Inhibition of tropiase occurred with leupeptin and N-bromosuccinimide, however, no inhibition was observed with α₂ -macroglobulin, soybean trypsin inhibitor, benzamidine-HCl or diisopropyl fluorophosphate.

収録刊行物

• 日本医真菌学会雑誌

  日本医真菌学会雑誌 48 (1), 19-25, 2007

  日本医真菌学会

キーワード

• <I>Candida tropicalis</I>
• acid proteinase
• purification

詳細情報

• CRID: 1390001204211119616
• NII論文ID: 130000067552
• NII書誌ID: AN10166867
• DOI: 10.3314/jjmm.48.19
• COI: 1:CAS:528:DC%2BD2sXktFertL0%3D
• ISSN: 18820476
• ISSN: 09164804
• NDL書誌ID: 023253361
• PubMed: 17287719
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023253361
• Web Site: https://search.jamas.or.jp/link/ui/2007287984
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可