Immunohistochemical Observation of Co-expression of E- and N-cadherins in Rat Organogenesis

• Sakamoto Atsushi

  Division of Forensic Medicine, Center for Community Medicine, Jichi Medical University School of Medicine

• Murata Kazumoto

  Division of Forensic Medicine, Center for Community Medicine, Jichi Medical University School of Medicine

• Suzuki Hideto

  Division of Forensic Medicine, Center for Community Medicine, Jichi Medical University School of Medicine

• Yatabe Megumi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

• Kikuchi Motoshi

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

抄録

Cadherins are a family of transmembrane glycoproteins that mediate cell-to-cell adhesion. Isoforms, including E- and N-cadherin, have been identified and shown to regulate morphogenesis through homophilic binding. In the ontogeny, the expressions of E- and N-cadherin change spatiotemporally, and the changes in cadherin isoforms, called cadherin switching, impact the mechanical adhesion of cells. Furthermore, cadherin functions as a receptor that transfers information from outside to inside cells, and in terms of switching, it affects cell phenotypes. To observe the expression patterns of E- and N-cadherins during embryogenesis and to identify cells that transiently coexpress both cadherins, we employed a recently developed immunohistochemical double staining technique in rat fetuses. At embryonic day 9, embryonic ectodermal cells more dominantly expressed E-cadherin, while mesodermal cells more dominantly expressed N-cadherin. At embryonic day 10, the expression pattern of E-cadherin in the surface ectoderm and endoderm and that of N-cadherin in the neuroectoderm were established. After embryonic day 10, unique co-expression of E- and N-cadherin was observed in primordia, such as the bulbus cordis, otic pit, notochord, and Rathke’s pouch. In the present study, it was possible to visualize the expression patterns of E- and N-cadherin during early fetal development, which enabled us to morphologically clarify cadherin switching.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 41 (5), 143-147, 2008

  日本組織細胞化学会

キーワード

• cadherin switching
• organogenesis
• immunohistochemistry
• rat

詳細情報

• CRID: 1390282679839044096
• NII論文ID: 130000070542
• DOI: 10.1267/ahc.08026
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/41/5/41_08026/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009159698
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可