Two Distinct Effects of cGMP on Cytosolic Ca2+ concentration of Rat Pancreatic β-Cells

• Ishikawa Tomohisa

  Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka

• Kaneko Yukiko

  Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka

• Sugino Fumi

  Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka

• Nakayama Koichi

  Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka

書誌事項

タイトル別名

• Two Distinct Effects of cGMP on Cytosolic Ca2+ Concentration of Rat Pancreatic .BETA.-Cells.
• Two Distinct Effects of cGMP on Cytosolic Ca2 concentration of Rat Pancreatic ベータ Cells

抄録

The present study investigated the effects of cGMP on cytosolic Ca²⁺ concentration ([Ca²⁺]_c) of isolated rat pancreatic β-cells. In the presence of 7.0 mM glucose, NOC 7, a nitric oxide (NO) donor, caused an increase in [Ca²⁺]_c of the β-cells, which was abolished by the soluble guanylate cyclase inhibitor ODQ. Similar [Ca²⁺]_c elevation was evoked by 8-bromo-cGMP. The [Ca²⁺]_c elevating responses to NOC 7 and 8-bromo-cGMP were abolished by nicardipine or in a Ca²⁺-free medium, but were not affected by thapsigargin, suggesting that they are produced by the Ca²⁺ influx through L-type voltage-operated Ca²⁺ channels. In contrast, NOC 7 and 8-bromo-cGMP decreased the [Ca²⁺]_c when it was raised in advance by the elevation of external K⁺ concentration to 30 mM or by 4-aminopyridine. The pretreatment with thapsigargin almost abolished the [Ca²⁺]_c reduction induced by the agents, suggesting that the action is likely to be primarily attributable to an acceleration of the Ca²⁺ sequestration into the endoplasmic reticulum. These results suggest that cGMP has two distinct effects on the [Ca²⁺]_c of rat pancreatic β-cells: a facilitation of the Ca²⁺ influx through L-type voltage-operated Ca²⁺ channels and an acceleration of the Ca²⁺ sequestration in the endoplasmic reticulum.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 91 (1), 41-46, 2003

  公益社団法人 日本薬理学会

キーワード

• islets of Langerhans
• calcium
• cGMP
• NOC 7
• glucose

詳細情報

• CRID: 1390001205176661632
• NII論文ID: 130000073727
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.91.41
• COI: 1:CAS:528:DC%2BD3sXhsFWgsLc%3D
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 6494495
• PubMed: 12686729
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6494495
• Web Site: https://search.jamas.or.jp/link/ui/2003183680
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可