The Action Mode of Lysophosphatidylcholine in Human Monocytes

• Yun Mi-Ran

  Laboratory of Pharmacology, College of Pharmacy, Pusan National University

• Okajima Fumikazu

  Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University

• Im Dong-Soon

  Laboratory of Pharmacology, College of Pharmacy, Pusan National University

書誌事項

タイトル別名

• Action Mode of Lysophosphatidylcholine in Human Monocytes

抄録

To elucidate the action and signal transduction of lysophosphatidylcholine (LPC), we challenged a set of LPC on U937 human monocytes and found that LPC mobilized Ca²⁺. The Ca²⁺ response was not blocked by pertussis toxin, an inhibitor of G_i/o proteins, or by U73122, a phospholipase C inhibitor. Furthermore, the response was totally blocked by addition of EGTA to the extracellular media, suggesting that Ca²⁺ influx across the plasma membrane was the only source of LPC-induced Ca²⁺ response in the U937 cells. 16:0 and 18:0 LPC induced similar responses. Recently it has been suggested that two G protein-coupled receptors function as LPC receptors in the plasma membrane. RT-PCR analysis indicated that neither the G2A receptor nor the GPR4 receptor is expressed in the U937 monocytes. Our data suggests that another action mechanism of LPC may be involved in the LPC response in the U937 cells.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 94 (1), 45-50, 2004

  公益社団法人 日本薬理学会

キーワード

• lysophosphatidylcholine
• atherosclerosis
• G protein-coupled receptor
• calcium influx
• signal transduction

詳細情報

• CRID: 1390282680153591168
• NII論文ID: 130000073882
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.94.45
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 6819207
• PubMed: 14745117
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6819207
• Web Site: https://search.jamas.or.jp/link/ui/2004120216
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可