Existence of Ionotropic Glutamate Receptor Subtypes in Cultured Rat Retinal Ganglion Cells Obtained by the Magnetic Cell Sorter Method and Inhibitory Effects of 20-Hydroxyecdysone, a Neurosteroid, on the Glutamate Response.
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- Mukai Satoshi
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Mishima Hiromu K.
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Shoge Keisuke
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Shinya Makoto
- Department of Ophthalmology, Hiroshima University School of Medicine
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- Ishihara Kumatoshi
- Department of Pharmacotherapy, Graduate School of Medical Sciences, Hiroshima University
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- Sasa Masashi
- Department of Pharmacology, Hiroshima University School of Medicine
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抄録
Glutamate and neurosteroids are known to exist in retinal ganglion cells (RGC). Therefore, patch clamp studies using the whole-cell recording method were performed to determine whether or not ionotropic glutamate receptor subtypes, i.e., N-methyl-<sc>D</sc>-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors, were present on RGC obtained by the magnetic cell sorter (MACS) method and cultures. In addition, the effects of 20-hydroxyecdysone (20-HE), a neurosteroid, on inward currents induced by NMDA, AMPA and kainate were examined at a holding potential of −60 mV. The current-voltage relationship for NMDA in the presence of glycine and Mg2+-free, as well as those for AMPA and kainate were linear, with a reversal potential of around 0 mV. NMDA-induced currents were blocked by MK-801, while both AMPA- and kainate-induced currents were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Application of 20-HE in the bath resulted in significant inhibitions on NMDA-, AMPA- and kainate-induced currents. Thus, NMDA, AMPA and kainate receptors were confirmed to exist on MACS-separated cultured RGC. Moreover, 20-HE inhibited NMDA receptor-mediated currents most prominently and AMPA- and kainate-mediated currents moderately, suggesting that neurosteroids may be playing a role in modulating glutamate-mediated transmission in RGC, and 20-HE might be useful for preventing glutamate neurotoxicity.
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 89 (1), 44-52, 2002
公益社団法人 日本薬理学会
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詳細情報
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- CRID
- 1390001204285988608
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- NII論文ID
- 130000078224
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- NII書誌ID
- AA00691188
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- COI
- 1:STN:280:DC%2BD38zktFSmtQ%3D%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 6169375
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- PubMed
- 12083742
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可