Changes of tissue factor dependent coagulant activity mediated by adhesion between polymorphonuclear cells and endothelial cells

• Watanabe Tohru

  Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

• Tokuyama Shogo

  Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

• Yasuda Masako

  Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

• Sasaki Tadanori

  Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

• Yamamoto Toshinori

  Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University

書誌事項

タイトル別名

• Changes of Tissue Factor-Dependent Coagulant Activity Mediated by Adhesion Between Polymorphonuclear Leukocytes and Endothelial Cells.

抄録

The purpose of this study was to examine whether polymorphonuclear leukocytes (PMNs) facilitate a tissue factor, a physiologic initiator of coagulation in endothelial cells, -dependent coagulant activity (TF activity). The TF activity in bovine endothelial cells (BAECs) was significantly increased in a concentration-dependent manner by PMNs (1 × 10⁵ - 1 × 10⁷ cells/ml) without affecting the treatment of N-formyl-methionyl-leucyl-phenylalanine, a selective activator of PMNs, and the addition of PMNs finally resulted in cell damage as evaluated by the lactate dehydrogenase leakage method. In the same conditions, an increase of adhesion between PMNs and BAECs was also observed in a time-dependent manner. However, since direct adhesion of PMNs to BAECs was impossible by using the transwell, PMNs failed to induce any changes in the TF activity. Hence, the change of TF activity found here might be closely related to the PMNs adhesion to BAECs. Indeed, anti-intercellular adhesion molecule-1 (anti-ICAM-1) antibody blocked the increase of TF activity in BAECs. These findings suggest that PMNs could increase TF activity in endothelial cells, which is triggered by adhesion to endothelial cells through ICAM-1.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 86 (4), 399-404, 2001

  公益社団法人 日本薬理学会

キーワード

• Endothelial cell
• Polymorphonuclear leukocyte
• Tissue factor
• Adhesion molecule

詳細情報

• CRID: 1390001204287765760
• NII論文ID: 130000078351
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.86.399
• COI: 1:CAS:528:DC%2BD3MXmtlCgsro%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 5885833
• PubMed: 11569613
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5885833
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819303257?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819303257?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2002077588
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可