Axonal and Dendritic Extension by Protopanaxadiol-Type Saponins From Ginseng Drugs in SK-N-SH Cells.

• Tohda Chihiro

  Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

• Matsumoto Noriaki

  Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

• Zou Kun

  Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

• Meselhy Meselhy R.

  Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

• Komatsu Katsuko

  Research Center for Ethnomedicines, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

抄録

Extension of axons and dendrites in neurons may compensate for and repair damaged neuronal networks in the dementia brain. To find out drugs capable of regenerating the neuronal network, we focused on several herbal drugs belonging to the genus Panax, kinds of Ginseng, and investigated neurite outgrowth activity of their extracts and compounds. We found that the methanol extracts of Ginseng (root of P. ginseng), Notoginseng (root of P. notoginseng) and Ye-Sanchi in Chinese (rhizome of a relative to P. vietnamensis) increased neurite outgrowth in SK-N-SH cells. The protopanaxadiol-type saponins, ginsenosides Rb₁ and Rb₃, and notoginsenosides R₄ and Fa isolated from Ye-Sanchi extract extended neurites, while protopanaxatriol-, ocotillol- and oleanane-type saponins had no effect on the neurite outgrowth. The percentage of cells with multipolar neurites and number of varicosities were intensely high in cells treated with the methanol extract of Ye-Sanchi as well as ginsenosides Rb₁ and Rb₃, and notoginsenosides R₄ and Fa. Both phosphorylated NF-H-expressing neurites and MAP2-expressing ones were extended by treatment with those saponins and the extract. Especially, longer neurites were mainly positive for phosphorylated NF-H. These results suggest that protopanaxadiol-type saponins enhance axonal and dendritic formation activity.<br>

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 90 (3), 254-262, 2002

  公益社団法人 日本薬理学会

キーワード

• Ginseng
• Protopanaxadiol saponin
• Axon
• Dendrite
• SK-N-SH cell

詳細情報

• CRID: 1390282679262151296
• NII論文ID: 130000078419
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.90.254
• COI: 1:STN:280:DC%2BD38jjslarsg%3D%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 6363070
• PubMed: 12499580
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6363070
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819300307?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819300307?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2003145564
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可