The mRNA Expression of Serotonin 2C Subtype Receptors Uncoupled With Inositol Hydrolysis in NG108-15 Cells.
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- Tohda Michihisa
- Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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- Sukma Monrudee
- Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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- Nomura Yasuyuki
- Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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- Watanabe Hiroshi
- Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
書誌事項
- タイトル別名
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- mRNA Expression of Serotonin 2C Subtype Receptors Uncoupled With Inositol Hydrolysis in NG108 15 Cells
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Cell culture systems seem to be useful for clarifying the cellular physiological mechanisms of serotonin 2C subtype receptors (5-HT2CR) and related drug action mechanisms. However, there are still few reports about cells that contain intrinsic 5-HT2CR. This report demonstrates by using RT/PCR that 5-HT2CR mRNA exists in splicing variant forms in NGI08-15 cells. The PCR results using a pair of primers that recognized sequences near the third intracellular loop site showed two neighboring bands at about 500 bp upon electrophoresis in acrylamide gels. The sequence analysis demonstrated that one band was the rat 5-HT2CR sequence and the other one was that of the mouse. Serotonin, however, did not enhance the inositol phosphates formation in NG108-15 cells. It has been reported that post-translational modifications of RNA, splicing and editing, occur at the site of the second intracellular loop domain in 5-HT2CR mRNA. Accordingly, a pair of primers that recognized this site were designed. The molecular size of the PCR product was shorter than that expected based on the sequence of the native 5-HT2CR. The fragment lacked the 95 nucleotides of native 5-HT2CR mRNA. This seems to be the reason why serotonin did not enhance inositol phosphates formation in NG108-15 cells.<br>
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 90 (2), 138-144, 2002
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204287771776
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- NII論文ID
- 130000078431
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- NII書誌ID
- AA00691188
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- COI
- 1:STN:280:DC%2BD38nmtVWisg%3D%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 6336826
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- PubMed
- 12419884
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可