High-density lipoprotein-associated α-tocopherol uptake by human retinal pigment epithelial cells (ARPE-19 cells): the irrelevance of scavenger receptor class B, type 1

• Akanuma Shin-ichi

  Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

• Yamamoto Atsushi

  Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

• Okayasu Shun

  Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

• Tachikawa Masanori

  Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

• Hosoya Ken-ichi

  Department of Pharmaceutics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

書誌事項

タイトル別名

• High-Density Lipoprotein-Associated .ALPHA.-Tocopherol Uptake by Human Retinal Pigment Epithelial Cells (ARPE-19 Cells): the Irrelevance of Scavenger Receptor Class B, Type I
• High density lipoprotein associated a tocopherol uptake by human retinal pigment epithelial cells ARPE 19 cells the irrelevance of scavenger receptor class B type 1

抄録

The purpose of this study was to determine the high-density lipoprotein (HDL)-associated α-tocopherol (α-tocopherol-HDL) transport and clarify the contribution of scavenger receptor class B, type I (SR-BI) to the uptake in the human retinal pigment epithelial cell line (ARPE-19 cells). [³H]α-Tocopherol-HDL uptake into ARPE-19 cells seeded onto a transwell from both the apical (apical-to-cell) and basal compartment (basal-to-cell) exhibited a time-dependent increase for 90 min and there was a reduction at 4 °C. These results indicate the involvement of carrier-mediated process in α-tocopherol-HDL uptake in ARPE-19 cells. Immunoblot analysis revealed that SR-BI protein was expressed in ARPE-19 cells. However, the uptake of [³H]α-tocopherol from the apical or basal compartment was hardly inhibited by block lipid transport-1 (BLT-1), a specific inhibitor of the SR-BI-mediated lipid transfer. In conclusion, ARPE-19 cells have a carrier-mediated transport mechanism of [³H]α-tocopherol-HDL regardless of any SR-BI-mediated process.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 32 (6), 1131-1134, 2009

  公益社団法人 日本薬学会

キーワード

• α-tocopherol
• outer blood-retinal barrier
• scavenger receptor class B type I

詳細情報

• CRID: 1390282679604806656
• NII論文ID: 130000117202
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.32.1131
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10235457
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10235457
• Web Site: http://www.jstage.jst.go.jp/article/bpb/32/6/32_6_1131/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009325470
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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