Paeonol Exerts Anti-angiogenic and Anti-metastatic Activities through Downmodulation of Akt Activation and Inactivation of Matrix Metalloproteinases

• Kim Seung-Ae

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Lee Hyo-Jeong

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Ahn Kwang Seok

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Lee Hyo-Jung

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Lee Eun-Ok

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Ahn Kyoo-Seok

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Choi Seung-Hoon

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Jung Soo-Jin

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

• Kim Ji Young

  Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyunghee University

• Baek Namin

  Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyunghee University

• Kim Sung-Hoon

  Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyunghee University

抄録

Paeonol (2′-hydroxy-4′-methoxyacetophenone) is known to possess anti-inflammatory and anti-proliferative activities. Recently there is evidence that anti-inflammatory agents may be useful in the setting of angiogenesis-related diseases. Thus in the present study the anti-angiogenic activity of paeonol and its mechanism were investigated in vitro and in vivo. Paeonol significantly inhibited proliferation of basic fibroblast growth factor (bFGF)-stimulated human umbilical vein endothelial cells (HUVECs). Paeonol also significantly inhibited migration and tube formation of bFGF-stimulated HUVECs in vitro. In addition, paeonol significantly suppressed neovessel formation on bFGF-treated chick chorioallantoic membrane (CAM) and disrupted bFGF-induced neovascularization in Matrigel plug assay in vivo. Furthermore, paeonol downregluated Akt phosphorylation in bFGF-stimulated HUVECs and reduced expression of matrix metalloproteinases-2 and -9 in HT1080 human fibrosarcoma cells. The Akt inhibitor LY294002 synergistically potentiated paeonol-induced inactivation of Akt and vascular endothelial growth factor in bFGF-treated HUVECs. Taken together, these findings suggest that paeonol can be a potent suppressor of angiogenesis and metastasis partially through inhibition of Akt signaling pathway and matrix metalloproteinase activity.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 32 (7), 1142-1147, 2009

  公益社団法人 日本薬学会

キーワード

• paeonol
• basic fibroblast growth factor
• human umbilical vein endothelial cell
• angiogenesis
• Akt

詳細情報

• CRID: 1390282679604072832
• NII論文ID: 130000117207
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.32.1142
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10264104
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10264104
• Web Site: http://www.jstage.jst.go.jp/article/bpb/32/7/32_7_1142/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009328356
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可