Displacement of Actin Filament and Actin Binding Proteins under Local Deformation Processed by Digital Image Correlation Method in a Myoblast

• MIZUTANI Takeomi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• DOI Kenichi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• MORITA Yasuyuki

  Research Institute for Applied Mechanics, Kyushu University

• UCHINO Masakazu

  Fukuoka Industrial Technology Center

• TODO Mitsugu

  Research Institute for Applied Mechanics, Kyushu University

• HAGA Hisashi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• KAWABATA Kazushige

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

抄録

Displacement of actin filament and its binding proteins in mouse myoblasts under locally applied deformation was analyzed by manual method or digital image correlation method. Cyotoskeletal components labeled by immunofluorescent technique or green fluorescent protein-fused protein were deformed via the movement of a glass needle which was poked into a cell. First, we confirmed the digital image correlation method is able to use to analyze displacement map by comparison of the manual method. Next, we examined whether the applied deformation isotropically propagates into cell body. At focal adhesions, fluorescent signals from the deformed area unchanged under the application. Mainly, focal adhesions around the poked area were moved to the direction of the movement of the needle. In addition, some adhesions away from the poked area were moved. Similar results were observed in phalloidin-stained cells. Finally, we applied the local deformation to live cells. However, displacement at the locally deformed area was not observed due to the disappearance of fluorescent signals. These results indicate that applied deformation propagated heterogeneously into a cell, and may imply that biochemical signals disrupt actin fibers under local deformation.

収録刊行物

• Journal of Biomechanical Science and Engineering

  Journal of Biomechanical Science and Engineering 4 (3), 415-422, 2009

  一般社団法人 日本機械学会

キーワード

• Mechanical Loading
• Digital Image Correlation Method
• Cytoskeletal Network
• Remodeling

詳細情報

• CRID: 1390001205261517696
• NII論文ID: 130000129016
• DOI: 10.1299/jbse.4.415
• ISSN: 18809863
• Web Site: http://www.jstage.jst.go.jp/article/jbse/4/3/4_3_415/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可