Priming Effects of Lipopolysaccharide and Inflammatory Cytokines on Canine Granulocytes
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- MAEDA Kenichi
- School of Veterinary Medicine, Kitasato University
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- SAKONJU Iwao
- School of Veterinary Medicine, Kitasato University
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- KANDA Aya
- School of Veterinary Medicine, Kitasato University
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- SUZUKI Tatsuya
- School of Veterinary Medicine, Kitasato University
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- KAKUTA Tomoko
- School of Veterinary Medicine, Kitasato University
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- SHIMAMURA Shunsuke
- School of Veterinary Medicine, Kitasato University
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- OKANO Shozo
- School of Veterinary Medicine, Kitasato University
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- TAKASE Katsuaki
- School of Veterinary Medicine, Kitasato University
Bibliographic Information
- Other Title
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- Surgery: Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes
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Abstract
Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor-α (rcTNF-α) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF-α and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF-α and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF-α having a priming effect similar to that observed in humans.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 72 (1), 55-60, 2010
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206428095232
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- NII Article ID
- 130000134511
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 10563691
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed