Proteomic analysis of the salivary glands of the rice brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae)

• Konishi Hirosato

  National Institute of Agrobiological Sciences

• Noda Hiroaki

  National Institute of Agrobiological Sciences

• Tamura Yasumori

  National Institute of Agrobiological Sciences

• Hattori Makoto

  National Institute of Agrobiological Sciences

書誌事項

タイトル別名

• Proteomic analysis of the salivary glands of the rice brown planthopper, Nilaparvata lugens (Stal) (Homoptera: Delphacidae)
• Proteomic analysis of the salivary glands of the rice brown planthopper, <italic>Nilaparvata lugens</italic> (Stål) (Homoptera: Delphacidae)

抄録

The rice brown planthopper, Nilaparvata lugens is a phloem-feeding insect pest of the rice plant Oryza sativa. To understand the role of saliva in its feeding process, we analyzed salivary gland proteins using two-dimensional polyacrylamide gel electrophoresis followed by Edman degradation of proteins. Of the 52 major spots analyzed, 25 N-terminal sequences were determined. The internal amino acid sequences of the remaining 27 proteins were determined by the Cleveland peptide mapping method. We identified 22 of the 25 proteins whose N-terminal regions were sequenced and 13 of the 27 proteins that were internally sequenced after searching them against the entries in the expressed sequence tag (EST) database of N. lugens. Through a homology search using public nonredundant protein databases, we identified EST-unmatched proteins. As a result, many housekeeping proteins responsible for energy metabolism, protein synthesis, folding and modification were identified, of which actins, tubulins, heat-shock proteins, protein disulfide isomerases and ATP synthases were in common with proteins identified from the salivary glands of hematophagous arthropods such as Anopheles gambiae, and a phytophagous gall midge Mayetiola destructor. However, we could not identify the glucosidases and phenoloxidases previously detected in the salivary glands of N. lugens. In contrast, novel proteins with an EF-hand domain were found in relatively high abundance in salivary gland homogenates derived from N. lugens; their corresponding ESTs were specifically derived from the salivary glands. Their calcium-binding properties suggest their importance of phloem feeding by the planthopper.

収録刊行物

• Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ

  Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ 44 (4), 525-534, 2009

  日本応用動物昆虫学会

キーワード

• Brown planthopper
• salivary gland
• proteome
• EF-hand domain

詳細情報

• CRID: 1390001206242301312
• NII論文ID: 130000142899
• NII書誌ID: AA00543238
• DOI: 10.1303/aez.2009.525
• ISSN: 1347605X
• ISSN: 00036862
• NDL書誌ID: 10449873
• Web Site: http://id.ndl.go.jp/bib/10449873
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10449873
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可