Isolation of Trypanosoma (Megatrypanum) theileri from Dairy Cattle in Taiwan

• LEE Yen-Feng

  Department of Medical Research and Education, Taichung Veterans General Hospital Department of Veterinary Medicine, National Chung-Hsing University

• CHENG Ching-Chang

  Department of Medical Research and Education, Taichung Veterans General Hospital Department of Veterinary Medicine, National Chung-Hsing University

• LIN Nai-Nu

  Department of Medical Research and Education, Taichung Veterans General Hospital Department of Veterinary Medicine, National Chung-Hsing University

• LIU Shih-An

  Department of Otolaryngology, Taichung Veterans General Hospital

• TUNG Kwong-Chung

  Department of Veterinary Medicine, National Chung-Hsing University

• CHIU Yung-Tsung

  Department of Medical Research and Education, Taichung Veterans General Hospital Department of Animal Science, National Chung-Hsing University

抄録

Although Trypanosoma (Megatrypanum) theileri, a blood parasite of bovid species, is spread widely throughout the world, it has never been reported in Taiwan. When an anti-coagulated blood sample from febrile dairy cattle was directly smeared, no parasite was observed. However, a highly distinctive morphological feature of trypanosome appeared in baby hamster kidney (BHK) cell culture inoculated with non-thrown blood buffy coat. The different stages and typical ultrastructures of trypanosome were observed in our isolate. The isolate was subsequently identified as T. theileri by species-specific PCR assay (Tth625), 18S rDNA sequencing alignment and internal transcribed spacer of ribosomal genes (ITS) as a marker for molecular phylogenetic analysis. The first T. theileri isolate in Taiwan (TWTth1) could be periodically passaged in BHK cell culture for more than one year and retained good re-cryopreservation viability. The BHK culture method would be excellent for diagnostic isolation and maintenance long-term development of this parasite.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 72 (4), 417-424, 2010

  公益社団法人 日本獣医学会

キーワード

• 18S rDNA
• cattle
• culture
• <i>T. theileri</i>

詳細情報

• CRID: 1390282681404757760
• NII論文ID: 130000149938
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.09-0343
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 10682962
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10682962
• Web Site: http://www.jstage.jst.go.jp/article/jvms/72/4/72_09-0343/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010265597
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可