Increase in the Neuraminidase Activity of a Nonpathogenic Newcastle Disease Virus Isolate during Passaging in Chickens

• TSUNEKUN Ryota

  Department of Veterinary Public Health, Faculty of Agriculture, Tottori University Avian Influenza Research Center, Kyoto Sangyo University

• ITO Hiroshi

  Department of Veterinary Public Health, Faculty of Agriculture, Tottori University Avian Zoonosis Reseach Center, Faculty of Agriculture, Tottori University

• KIDA Hiroshi

  Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

• OTSUKI Koichi

  Avian Influenza Research Center, Kyoto Sangyo University

• ITO Toshihiro

  Department of Veterinary Public Health, Faculty of Agriculture, Tottori University Avian Zoonosis Reseach Center, Faculty of Agriculture, Tottori University

抄録

A pathogenic mutant of the Newcastle disease virus (NDV) was previously generated by passaging a non-pathogenic isolate from wild waterfowl. Velogenic mutant 9a5b (IVPI=2.67) contains three amino acid substitutions (128H, 495K and 573stop) in the hemagglutinin-neuraminidase (HN) protein, as compared with nonpathogenic waterfowl isolate 415/91 strain, and two of these (128H and 495K) were introduced after mesogenic 9a3b (IVPI=1.88). To investigate the role of the HN protein in NDV virulence, the function of HN protein such as neuraminidase (NA), Hemadsorption (HAd) and fusion promotion activities was examined by introducing the point mutations observed in passaged mutants into the HN gene cDNAs. In vitro functional assay using mutant protein expression demonstrated that the 128H substitution markedly increases NA activity and 573stop substitution increase NA and HAd activities. On the other hand, 495K substitution had little effect on any activities. These results indicate that a single amino acid substitution (128P to H) in the NDV HN protein affects the neuraminidase activity and is possibly correlated with the virulence.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 72 (4), 453-457, 2010

  公益社団法人 日本獣医学会

キーワード

• hemagglutinin-neuraminidase protein
• IVPI
• Newcastle disease virus
• passage
• pathogenicity

詳細情報

• CRID: 1390282681404797184
• NII論文ID: 130000149945
• NII論文ID: 40017112797
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.09-0474
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 10683037
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10683037
• Web Site: http://www.jstage.jst.go.jp/article/jvms/72/4/72_09-0474/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可