Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

• KONISHI Tomoyuki

  Forestry and Forest Products Research Institute

• MIYAZAKI Yasumasa

  Forestry and Forest Products Research Institute

• YAMAKAWA Seiyei

  University of Tsukuba

• IWAI Hiroaki

  University of Tsukuba

• SATOH Shinobu

  University of Tsukuba

• ISHII Tadashi

  Forestry and Forest Products Research Institute

抄録

Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (1), 191-194, 2010

  公益社団法人 日本農芸化学会

キーワード

• arabinofuranose
• cell-wall biosynthesis
• reversibly glycosylated polypeptides
• recombinant proteins
• UDP-arabinopyranose mutase

詳細情報

• CRID: 1390282681455131392
• NII論文ID: 10027550650
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90619
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10533150
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10533150
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90619
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可