High performance liquid chromatographic determination of clarithromycin in lymphocytes using a post-column with tris(2,2'-bipyridine) ruthenium (3) chemiluminescence detection
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- Chiba Ryoko
- Showa Pharmaceutical University
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- Sokura Makiko
- Showa Pharmaceutical University
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- Ikejima Hideaki
- Showa Pharmaceutical University
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- Hoshi Keiko
- Showa Pharmaceutical University
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- Hamashima Hajime
- Showa Pharmaceutical University
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- Inoue Yoshihiro
- Showa Pharmaceutical University
書誌事項
- タイトル別名
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- High Performance Liquid Chromatographic Determination of Clarithromycin in Lymphocytes Using a Post-column with Tris(2,2'-bipyridine) Ruthenium (III) Chemiluminescence Detection
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The concentration of antibiotics in cells must be determined for effective treatment of infectious diseases caused by obligate intracellular parasites, such as Chlamydia trachomatis and Legionella pneumophila. We confirmed the usefulness of high performance liquid chromatography (HPLC), which is already commonly used in medical facilities, for the measurement of antibiotics in cells. The measurement was carried out using a post-column with tris(2,2'-bipyridine) ruthenium (III) chemiluminescence detection. Clarithromycin (CAM) was used as the model antibiotic. The retention time of CAM on the column was 7.6±0.4 min and the detection limit was 2.0×10-3 ng on column. The linearity of the calibration curve was guaranteed until 2.0 ng on column. This level of performance is comparable to that of liquid chromatography-tandem mass spectrometry. The system was able to monitor changes in the concentration of CAM in lymphocytes. These findings suggest that HPLC, a general-purpose, already widely used detection system, could also contribute to the development of effective individual antibiotic treatment regimens at a wide variety of medical facilities.
収録刊行物
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- JOURNAL OF HEALTH SCIENCE
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JOURNAL OF HEALTH SCIENCE 56 (1), 14-19, 2010
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204497069184
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- NII論文ID
- 130000157698
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- NII書誌ID
- AA11316464
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- ISSN
- 13475207
- 13449702
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- NDL書誌ID
- 10537127
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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