Bacteriology: Distribution of virulence genes related to adhesions and toxins in Shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan

• WU Yuluo

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• HINENOYA Atsushi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• TAGUCHI Takashi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• NAGITA Akira

  Department of Pediatrics, Mizushima Central Hospital

• SHIMA Kensuke

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• TSUKAMOTO Teizo

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• SUGIMOTO Norihiko

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• ASAKURA Masahiro

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• YAMASAKI Shinji

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

書誌事項

タイトル別名

• Distribution of Virulence Genes Related to Adhesions and Toxins in Shiga Toxin-Producing Escherichia coli Strains Isolated from Healthy Cattle and Diarrheal Patients in Japan
• Distribution of virulence genes related to adhesins and toxins in Shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan

抄録

Shiga toxin-producing Escherichia coli (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., eae (intimin, E. coli attaching and effacing), saa (STEC autoagglutinating adhesin), iha (irgA homologue adhesin), efa1 (E. coli factor for adherence), lpfA_O113 (long polar fimbriae), and ehaA (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic cdt (cytolethal distending toxin), and subAB (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for lpfA_O113 (99%), ehaA (95%), iha (94%), saa (91%), subAB (65%), and cdt-V (49%), respectively, while 2 were positive for eae (1.2%) and efa1 (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for ehaA (98%), iha (97%), efa1 (95%), and eae (95%), respectively, while 11, 2, 2, and 1 were positive for lpfA_O113 (18%), saa (3.3%), cdt-V (3.3%), and subAB (1.6%), respectively. Therefore, in human STEC isolates efa1 and eae whereas in cattle isolates saa, lpfA_O113, cdt-V and subAB were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 72 (5), 589-597, 2010

  公益社団法人 日本獣医学会

キーワード

• bacterial toxi
• cattle
• <i>E. coli</i> infection
• molecular epidemiology
• virulence genes

詳細情報

• CRID: 1390001206428366336
• NII論文ID: 130000168009
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.09-0557
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 10714656
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10714656
• Web Site: http://www.jstage.jst.go.jp/article/jvms/72/5/72_09-0557/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可