Mechanism of Inhibitory Effect of Prednisolone on RhoA Upregulation in Human Bronchial Smooth Muscle Cells
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- Goto Kumiko
- Department of Pharmacology, School of Pharmacy, Hoshi University
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- Chiba Yoshihiko
- Department of Pharmacology, School of Pharmacy, Hoshi University
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- Sakai Hiroyasu
- Department of Pharmacology, School of Pharmacy, Hoshi University
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- Misawa Miwa
- Department of Pharmacology, School of Pharmacy, Hoshi University
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RhoA plays an important role in Ca2+ sensitization of bronchial smooth muscle in antigen-induced airway hyperresponsiveness (AHR). Glucocorticoids are now the most effective anti-inflammatory treatment for asthma, and inhaled corticosteroids are the most effective long-term control therapy for persistent asthma. To determine the mechanism of the inhibitory action of glucocorticoids on AHR in allergic bronchial asthma, that of prednisolone on RhoA upregulation was investigated using cultured human bronchial smooth muscle cells (hBSMCs). The upregulation of RhoA induced by interleukin (IL)-13 and tumor necrosis factor (TNF)-α, major mediators for development of AHR, was observed in hBSMCs. Prednisolone partly inhibited the IL-13-induced RhoA upregulation and RhoA promoter activity, although prednisolone had no effects on the activations of signal transducers and activators of transcription (STAT)6 and nuclear factor (NF)-κB. Increased expression and promoter activity of RhoA induced by TNF-α was completely inhibited by prednisolone, although the activation of NF-κB failed to be inhibited by prednisolone in hBSMCs. These findings suggest that prednisolone might inhibit NF-κB-induced transcription via interaction between glucocorticoid receptor (GR), resulting in an inhibition of RhoA upregulation induced by IL-13 and TNF-α.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 33 (4), 710-713, 2010
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679604832384
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- NII論文ID
- 130000248024
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 10622944
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- KAKEN
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- 使用不可