LC Determination and Pharmacokinetic Study of Hyperoside in Rat Plasma after Intravenous Administration

• LIU Xun

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• WANG Dong

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• WANG Si-Yuan

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• MENG Xian-Sheng

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• ZHANG Wen-Jie

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• YING Xi-Xiang

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

• KANG Ting-Guo

  School of Pharmacy, Liaoning University of Traditional Chinese Medicine

抄録

  A simple and specific high-performance liquid chromatographic (HPLC) method was developed for the pharmacokinetic study of hyperoside (HP, isolated from the leaves of Crataegus pinnatifida Bge. var. major) in rats after intravenous administration. The plasma samples were deproteinized with methanol after addition of internal standard (I.S.), baicalin. HPLC analysis was performed on a Diamonsil C18 analytical column, using methanol-0.6% aqueous phosphoric acid (45:55, v/v) as the mobile phase with UV detection at 340 nm. The calibration curve was linear over the range of 0.8921-59.7125 μg/ml in rat plasma. The average extraction recovery of HP was 99.33±0.86%, and the relative standard deviations (R.S.D.s) of the intra- and inter-day precisions were no more than 7.8 and 2.5%, respectively. The lower limit of quantification (LLOQ) was 0.8921 μg/ml. The validated method was successfully applied during a pharmacokinetic study in rats after intravenous administration of HP at different doses, and all the results indicated that the pharmacokinetics of HP in rats obeyed nonlinear processes.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 130 (6), 873-879, 2010-06-01

  公益社団法人 日本薬学会

キーワード

• hyperoside
• pharmacokinetics
• HPLC
• rat plasma

詳細情報

• CRID: 1390282681103837952
• NII論文ID: 130000259480
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.130.873
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 10725925
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10725925
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/130/6/130_6_873/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010329174
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可