Molecular Cloning of Canine Excitatory Amino Acid Transporter 5 and Its Detection in Primary Lens Epithelial Cells

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  • OCHIAI Hideharu
    Research Institute of Biosciences, Azabu University, School of Veterinary Medicine
  • SAITO Miyoko
    Laboratory of Veterinary Surgery 2, Azabu University, School of Veterinary Medicine
  • MARUO Takuya
    Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine
  • KANEMAKI Nobuyuki
    Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine

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The full-length cDNA sequence of canine excitatory amino acid transporter (EAAT) 5 was determined in samples taken from the canine retina. The sequence was 2,467 bp long and was predicted to encode the 560 amino acid polypeptides. The deduced amino acid sequence of canine EAAT5 showed similarities of 91.8 and 92.7% to those of humans and rats, respectively. In canine, EAAT5 has a 49.4% identity with EAAT1, 43.7% with EAAT2, 46.4% with EAAT3, and 45.7% with EAAT4. RT-PCR analysis revealed EAAT5 expression in primary lens epithelial cell culture and the cerebellum, and Western blot analysis detected a single band of 60 kDa which confirmed EAAT5 protein expression in these cells. In addition, all subtypes of EAAT were detected in canine lens epithelial cells, indicating the pivotal role of EAATs in supplying glutamate, the precursor of antioxidant glutathione in the lens.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 59 (4), 449-457, 2010

    公益社団法人 日本実験動物学会

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