Musca domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells through a Mitochondria-Mediated Reactive Oxygen Species Way

• Zhao Qi

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

• Cao Xiaohong

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

• Zeng Bin

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

• Wang Chunling

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

• Yan Le

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

• Xu Chengjian

  Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology

Abstract

A lectin from Musca domestica larva was purified by affinity chromatography on a glactose-Sepharose 4B column. Musca domestica larva lectin (MLL) inhibited the growth of BEL-7402 cells in a time and concentration-dependent way. The results of Hoechst 33258 staining indicated that MLL induce BEL-7402 cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that MLL treatment abrogated glutathione antioxidant system and induced mitochondria to generate reactive oxygen species (ROS) accumulation, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of Bax, the downregulation of Bcl-2, the cytochrome c release and the activation of the caspases pathways.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 33 (8), 1274-1278, 2010

  The Pharmaceutical Society of Japan

Keywords

• lectin
• <i>Musca domestica</i> larva
• antitumor
• apoptosis
• mitochondrial path

Details

• CRID: 1390001204627067776
• NII Article ID: 130000300347
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.33.1274
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 10764432
• Web Site: http://id.ndl.go.jp/bib/10764432
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10764432
• Web Site: http://www.jstage.jst.go.jp/article/bpb/33/8/33_8_1274/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011107837
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed