Thiamine ameliorates diabetes-induced inhibition of pyruvate dehydrogenase (PDH) in rat heart mitochondria: investigating the discrepancy between PDH activity and PDH E1α phosphorylation in cardiac fibroblasts exposed to high glucose
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- Kohda Yuka
- Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Japan
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- Umeki Masashi
- Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Japan
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- Kono Tatsuji
- Third Division, Department of Internal Medicine, Osaka Medical College, Japan
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- Terasaki Fumio
- Third Division, Department of Internal Medicine, Osaka Medical College, Japan
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- Matsumura Hitoshi
- Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Japan
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- Tanaka Takao
- Laboratory of Pharmacotherapy, Osaka University of Pharmaceutical Sciences, Japan
書誌事項
- タイトル別名
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- Thiamine Ameliorates Diabetes-Induced Inhibition of Pyruvate Dehydrogenase (PDH) in Rat Heart Mitochondria: Investigating the Discrepancy Between PDH Activity and PDH E1<I>α</I> Phosphorylation in Cardiac Fibroblasts Exposed to High Glucose
- Thiamine ameliorates diabetes induced inhibition of pyruvate dehydrogenase PDH in rat heart mitochondria investigating the discrepancy between PDH activity and PDH E1 a phosphorylation in cardiac fibroblasts exposed to high glucose
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The activity of pyruvate dehydrogenase (PDH) is reduced in diabetic patients. Phosphorylation of the PDH E1α subunit by PDH kinase contributes to the suppression of PDH activity. PDH requires thiamine as a coenzyme. We investigated the exact mechanism of diabetes-induced PDH inhibition, and the effect of thiamine in both in vivo and in vitro experiments. Treatment of rats with thiamine significantly, although partially, recovered streptozotocin (STZ)-induced reductions in mitochondrial PDH activity. Nevertheless, we found that PDH E1α phosphorylation in the thiamine-treated STZ group was perfectly diminished to the same level as that in the control group. STZ treatment significantly caused enhancements of the expression of O-glycosylated protein in the rat hearts, which was decreased by thiamine repletion. Next, the rat cardiac fibroblasts (RCFs) were cultured in the presence of high glucose levels. Thiamine dramatically recovered high glucose–induced PDH inhibition. High glucose loads did not alter the phosphorylated PDH E1α. PDH inhibition in RCFs was not accompanied by an increase in the PDH E1α phosphorylation. The O-glycosylated protein was markedly increased in RCFs exposed to high glucose, which was inhibited by thiamine. These results suggest that thiamine ameliorates diabetes-induced PDH inhibition by suppressing the increased expression of the O-glycosylated protein. The O-glycosylation of PDH E1α may be involved in the regulation of the PDH activity.
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 113 (4), 343-352, 2010
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205179608448
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- NII論文ID
- 10027746038
- 130000301211
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- NII書誌ID
- AA11806667
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 10790595
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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