Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics

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  • Tanthanuch Waraporn
    Synchrotron Light Research Institute (Public Organization)
  • Tittabutr Panlada
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
  • Mohammed Shabaz
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
  • Matthiesen Rune
    Bioinformatics Unit—CIC bioGUNE, Parque Tecnológico de Bizkaia Edificio
  • Yamabhai Montarop
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
  • Manassila Monchai
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
  • Jensen Ole Noerregaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark
  • Boonkerd Nantakorn
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
  • Teaumroong Neung
    School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology

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Abstract

Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 25 (4), 275-280, 2010

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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