Biodegradation Characteristics of Pharmaceutical Substances by Whole Fungal Culture Trametes versicolor and its Laccase
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- TRAN Ngoc Han
- Department of Civil and Environmental Engineering, Tokyo Institute of Technology, Japan
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- URASE Taro
- School of Bioscience and Biotechnology, Tokyo University of Technology, Japan
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- KUSAKABE Osamu
- Department of Civil and Environmental Engineering, Tokyo Institute of Technology, Japan
抄録
The degradation of 10 selected pharmaceutically active compounds (PhACs) by whole fungal culture Trametes versicolor, culture filtrates and commercial laccase preparation was conducted. Complete removal of diclofenac (DCF), naproxen (NPX), indomethacin (IDM), ibuprofen (IBP), and fenoprofen (FEP) and partial degradation of other selected PhACs were observed after 48 hours of incubation with the 7-day-old liquid fungal culture both in the presence and absence of ABTS (2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)) as a laccase-mediator. The catalytic activity of laccase in the degradation of selected PhACs was examined for both crude and commercial extracellular laccase preparations. The results showed that laccase preferentially removed DCF, NPX and IDM among the target PhACs removed by the whole fungal culture. Intracellular enzymes may be involved in the degradation of ketoprofen (KEP), clofibric acid (CA), carbamazepine (CBZ), propyphenazone (PPZ), fenoprofen (FEP) and gemfibrozil (GFZ). The removal of most selected PhACs was increased with the increase in laccase activity. The presence of redox mediators such as ABTS and HBT (1-hyroxybenzotriazole) promoted the degradation of selected PhACs, in which complete removal of DCF, NPX and IDM was observed after 3 hours of incubation with laccase activity (2000 U/L) in the presence of ABTS/HBT. The degradation spectrum by laccase for ionic PhACs with nitrogen-containing structure was quite different from that of the activated sludge process.
収録刊行物
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- Journal of Water and Environment Technology
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Journal of Water and Environment Technology 8 (2), 125-140, 2010
公益社団法人 日本水環境学会
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詳細情報 詳細情報について
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- CRID
- 1390282680156390912
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- NII論文ID
- 130000307735
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- ISSN
- 13482165
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可