Inhibitory effects of coumarins from the stem barks of Fraxinus rhynchophylla on adipocyte differentiation in 3T3-L1 cells
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- Shin Eunjin
- College of Pharmacy, Chungbuk National University
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- Choi Kyeong-Mi
- College of Pharmacy, Chungbuk National University
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- Yoo Hwan-Soo
- College of Pharmacy, Chungbuk National University
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- Lee Chong-Kil
- College of Pharmacy, Chungbuk National University
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- Hwang Bang Yeon
- College of Pharmacy, Chungbuk National University
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- Lee Mi Kyeong
- College of Pharmacy, Chungbuk National University
書誌事項
- タイトル別名
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- Inhibitory Effects of Coumarins from the Stem Barks of <i>Fraxinus rhynchophylla</i> on Adipocyte Differentiation in 3T3-L1 Cells
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In the course of screening anti-adipogenic activity of natural products employing the preadipocyte cell line, 3T3-L1 as an in vitro assay system, the EtOAc fraction of the stem barks of Fraxinus rhynchophylla DENCE (Oleaceae) showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of six coumarins such as esculetin (1), scopoletin (2), fraxetin (3), fraxidin (4) esculin (5) and fraxin (6). Among the six coumarins isolated, esculetin (1) showed the most potent inhibitory activity on adipocyte differentiation, followed by fraxetin (3). Further studies with interval treatment demonstrated that esculetin (1) exerted inhibitory activity on adipocyte differentiation when treated within 2 d (days 0—2) after differentiation induction. We further investigated the effect of esculetin (1) on peroxisome proliferator activated receptor γ (PPARγ), one of the early adipogenic transcription factors. Esculetin (1) significantly blocked the induction of PPARγ protein expression and inhibited adipocyte differentiation induced by troglitazone, a PPARγ agonist. Taken together, these results suggest that esculetin (1), an active compound from F. rhynchophylla, inhibited early stage of adipogenic differentiation, in part, via inhibition of PPARγ-dependent pathway.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 33 (9), 1610-1614, 2010
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204626731776
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- NII論文ID
- 130000322365
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 10797145
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可