Time-Lapse Videomicrographic Observations of Blastocyst Hatching in Cattle

• NIIMURA Sueo

  Faculty of Agriculture, Niigata University

• OGATA Tadahiro

  Graduate School of Science and Technology, Niigata University

• OKIMURA Ayano

  Faculty of Agriculture, Niigata University

• SATO Taro

  Livestock Research Center, Niigata Agricultural Research Institute

• UCHIYAMA Yasuhiko

  Livestock Research Center, Niigata Agricultural Research Institute

• SETA Takeshi

  Livestock Research Center, Niigata Agricultural Research Institute

• NAKAGAWA Hiroshi

  Livestock Research Center, Niigata Agricultural Research Institute

• NAKAGAWA Kuniaki

  Livestock Research Center, Niigata Agricultural Research Institute

• TAMURA Yuichi

  Livestock Research Center, Niigata Agricultural Research Institute

抄録

Morphological changes of cultured bovine blastocysts during hatching were observed using time-lapse videomicrography in order to investigate the patterns of the hatching process that occurred in the blastocysts and to determine whether the hatching patterns differed between blastocysts developed from fresh and cryopreserved embryos. Compacted morulae (CMs) were collected from superovulation-treated Japanese Black and Holstein dairy cattle and cultured in a medium in a CO₂ culture chamber equipped with an inverted microscope at 38.5 C. Images of resultant blastocysts during the period from blastocoel formation to completion of hatching were taken at 4-sec intervals by a CCD color camera connected to an inverted microscope and recorded by a time-lapse video cassette recorder. In blastocysts developed from fresh CMs, hatching was found to begin with protrusion of trophectoderm cells from zonae pellucidae at the expanded stage. Protrusion of the cells occurred in any site of the trophectoderm. After protrusion, a large or small slit was formed in the zona pellucida in all blastocysts as a result of blastocyst expansion or enlargement of the protrusion. Then, blastocysts completely escaped from the zona pellucida through the slit in the state of expansion. From these findings, the hatching patterns of cattle blastocysts could be classified into 5 types. In blastocysts developed from frozen-thawed CMs, the hatching pattern and length of time needed for hatching are similar to those in blastocysts developed from fresh CMs. In addition, the pregnancy rate of recipients following transfer of frozen-thawed CMs (52.4%) did not differ from that of recipients following transfer with fresh CMs (58.3%). These results suggested that the quality of frozen-thawed cattle embryos is comparable to that of fresh embryos and that there could be a relationship between the hatching pattern of blastocysts and the viability of embryos after transfer.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 56 (6), 649-654, 2010

  公益社団法人 日本繁殖生物学会

キーワード

• Cattle blastocyst
• Cryopreserved embryo
• Fresh embryo
• Hatching pattern
• Time-lapse videomicrography

詳細情報

• CRID: 1390001206335188992
• NII論文ID: 10027730414
• NII論文ID: 130000329056
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.10-069h
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 10931271
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10931271
• Web Site: http://www.jstage.jst.go.jp/article/jrd/56/6/56_10-069H/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011150174
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可