Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type 2 5α-reductase in vitro

• WANG Xingsheng

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

• LIAO Jianmin

  School of Life Science and Technology, China Pharmaceutical University

• YIN Dengke

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

• ZHAN Fan

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

• DAI Sufei

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

• XIE Guangyan

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

• SANG Xiaobing

  Department of Pharmacy, Anhui University of Traditional Chinese Medicine

書誌事項

タイトル別名

• Establishment of a Novel Model for Studying the Effects of Extracts of Chinese Herb Medicine on Human Type II 5α-Reductase <i>in Vitro</i>
• Establishment of a novel model for studying the effects of extracts of Chinese herb medicine on human type 2 5 a reductase in vitro

抄録

  Human steroid 5α-reductase type II (hSRD5A2) and dihydrotestosterone (DHT) play important roles in benign prostatic hyperplasia (BPH). The aim of our study was to establish a novel model to investigate the inhibitory effects of extracts and compounds of Chinese herb medicine on hSRD5A2. The gene, hSRD5A2, was artificially synthesized and cloned into pcDNA3.1(+) vector, which was transfected into CHO cells by liposome. Transfected cells were screened through G418 and MTX. The expressed protein of hSRD5A2 by cells was purified and detected by western blotting. A minim reactive system comprising hSRD5A2 and testosterone (T) as substrate together with NADPH as hydrogen donor was established for screening inhibitors of hSRD5A2. The reaction system was optimized in the concentrations of T, NADPH, and hSRD5A2 and reaction temperature, time, and activity of hSRD5A2 were determined by the production of DHT. Furthermore, we screened some extracts and compounds of Chinese herb medicine using this model. The concentrations of T, NADPH, and hSRD5A2 were 0.02 μM, 0.8 mM, and 0.05 U/μl, respectively, in the model; maximum activity of hSRD5A2 was achieved at 37°C and 60 min reaction, and mangiferin had significant inhibitory effect on the activity of hSRD5A2. The model in this study is convenient and reliable for screening and evaluation of inhibitors of hSRD5A2; mangiferin may be a potential medicine for the treatment of BPH.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 130 (9), 1207-1214, 2010-09-01

  公益社団法人 日本薬学会

キーワード

• human steroid 5α-reductase type II purification
• screening model
• inhibitor
• mangiferin

詳細情報

• CRID: 1390282681105215744
• NII論文ID: 130000342381
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.130.1207
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 10807207
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10807207
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/130/9/130_9_1207/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010341127
• 本文言語コード: en
• データソース種別:
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  • NDL
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