Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
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- Yamauchi Yoshinori
- Section of Orthodontics, Department of Oral Growth & Development, Division of Clinical Dentistry, Fukuoka Dental College
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- Tsuruga Eichi
- Section of Functional Structure, Department of Morphological Biology, Division of Biomedical Sciences, Fukuoka Dental College
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- Nakashima Kazuki
- Section of Orthodontics, Department of Oral Growth & Development, Division of Clinical Dentistry, Fukuoka Dental College
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- Sawa Yoshihiko
- Section of Functional Structure, Department of Morphological Biology, Division of Biomedical Sciences, Fukuoka Dental College
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- Ishikawa Hiroyuki
- Section of Orthodontics, Department of Oral Growth & Development, Division of Clinical Dentistry, Fukuoka Dental College
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抄録
Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropoelastin deposition. Among the fibulin family, fibulin-2, -4 and -5 are capable of binding to tropoelastin and fibrillin-1. In the present study, we used the RNA interference (RNAi) technique to establish individual gene-specific knockdown of fibulin-2, -4 and -5 in elastin-producing cells (human gingival fibroblasts; HGF). We then examined the extracellular deposition of tropoelastin using immunofluorescence. RNAi-mediated down-regulation of fibulin-4 and -5 was responsible for the diminution of tropoelastin deposition. Suppression of fibulin-5 appeared to inhibit the formation of fibrillin-1 microfibrils, while that of fibulin-4 did not. Similar results to those for HGF were obtained with human dermal fibroblasts. These results suggest that fibulin-4 and -5 may be associated in different ways with the extracellular deposition of tropoelastin during elastic fiber formation in elastin-producing cells in culture.<br>
収録刊行物
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- Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ
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Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 43 (6), 131-138, 2010
日本組織細胞化学会