Effects of Magnesium Sulphate Administration on Aquaporin 3 in Rat Gastrointestinal Tract

• Ikarashi Nobutomo

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Ushiki Takashi

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Mochizuki Toshihide

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Toda Takahiro

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Kudo Toshiyuki

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Baba Kohta

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Ishii Makoto

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Ito Kiyomi

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Ochiai Wataru

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

• Sugiyama Kiyoshi

  Department of Clinical Pharmacokinetics, School of Pharmacy and Pharmaceutical Sciences, Hoshi University

抄録

Aquaporin (AQP) 3 plays an important role in regulating faecal water content in the colon. We investigated the role of AQP3 in the colon in the laxative effect of magnesium sulphate (MgSO₄), a widely used osmotic laxative. Rats were administered MgSO₄, after which faecal water content, the colon mRNA expression levels of sodium myo-inositol transporter (SMIT) and taurine transporter (TauT), the colon protein expression levels of AQP3 were examined. Faecal water content increased over time after MgSO₄ administration, and severe diarrhoea was observed between 4 and 8 h after administration. The mRNA expression levels of SMIT and TauT, which are indicators of variations in osmotic pressure, were highest at 2 h after the administration of MgSO₄ and were still elevated at 8 h after administration when compared to immediately after the administration. The immunostaining analysis showed that AQP3 is a dominant AQP in the rat colon. The protein expression levels of AQP3 in the colon increased over time following the administration of MgSO₄ and at 8 h after administration were approximately 8 times higher than baseline levels. Previously, osmotic laxatives were believed to induce diarrhoea by elevating the osmotic pressure in the intestinal tract. The results of the present study suggest that the laxative effect of MgSO₄ is not simply caused by a change in the osmotic pressure in the intestinal tract, but could be a response to increased expression of AQP3.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (2), 238-242, 2011

  公益社団法人 日本薬学会

キーワード

• aquaporin
• magnesium sulphate
• laxative
• water channel
• sodium <i>myo</i>-inositol transporter
• taurine transporter

詳細情報

• CRID: 1390282679604129920
• NII論文ID: 130000402196
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.238
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10952353
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10952353
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/2/34_2_238/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011303495
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可