Development and application of a method to investigate drug-metabolizing enzyme inhibitors using sparteine for probe of cytochrome P450 2D6 and tris(2,2'-bipyridine)ruthenium(2)-electrogenerated chemiluminescence detection

• Shibano Yuu

  College of Pharmacy, Nihon University

• Taki Shiwori

  College of Pharmacy, Nihon University

• Miyamoto Aoi

  College of Pharmacy, Nihon University

• Uchikura Kazuo

  College of Pharmacy, Nihon University

書誌事項

タイトル別名

• Development and Application of a Method to Investigate Drug-Metabolizing Enzyme Inhibitors Using Sparteine for Probe of Cytochrome P450 2D6 and Tris(2,2'-bipyridine)ruthenium(II)-Electrogenerated Chemiluminescence Detection

抄録

We studied the detection of drug-metabolizing enzyme inhibitiors using column-switching high performance liquid chromatography with tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)₃²⁺)-electrogenerated chemiluminescence detection. This can be applied to evaluate the genetic diversity concerning the ability of cytochrome P450 (CYP) 2D6 to metabolize drug in vitro. We demonstrated the ability of CYP2D6 to enable us to examine drugs metabolizing enzyme inhibition with high performance and sensitivity. This method can be applied to investigate metabolite inhibitors of CYP2D6 in vitro and in vivo. Thus, Metixene was found to be a potential CYP2D6 inhibitor.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 59 (2), 249-253, 2011

  公益社団法人 日本薬学会

キーワード

• tris(2,2′-bipyridine)ruthenium(II)
• column-switching HPLC
• cytochrome P450 2D6
• Metixene

詳細情報

• CRID: 1390001204173102464
• NII論文ID: 130000405523
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.59.249
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 10951902
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10951902
• Web Site: http://www.jstage.jst.go.jp/article/cpb/59/2/59_2_249/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011335815
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可