Formulation and Pharmacokinetic Evaluation of Tetracycline-Loaded Solid Lipid Nanoparticles for Subcutaneous Injection in Mice

• Xu Xi-ming

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

• Wang Yan-song

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

• Chen Rong-ying

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

• Feng Chun-lai

  Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University

• Yao Feng

  Department of Surgery, Harvard Medical School, Brigham and Women's Hospital

• Tong Shan-shan

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

• Wang Li

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

• Yamashita Fumiyoshi

  Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University

• Yu Jiang-nan

  Department of Pharmaceutics, School of Pharmacy, Jiangsu University

抄録

The aim of this work was to prepare tetracycline-loaded solid lipid nanoparticles (Tet-SLN), and to evaluate the potential of these colloidal carriers for subcutaneous injection. Tet-SLN was prepared by microemulsion method and the preparation conditions were optimized by ternary phase diagram. At optimized process conditions, lyophilized Tet-SLN showed spherical particles with a mean diameter of 87.2±46.9 nm and a negative zeta potential of −6.69 mV, up to 1.7% tetracycline drug content was achieved after loading. In vitro release test showed a biphasic release profile for Tet-SLN and more than 80% of the drug was liberated from Tet-SLN in 48 h. After subcutaneous injection of Tet-SLN to mice, a considerable sustained release was observed; tetracycline in blood could be detected lasting 36 h, and lower concentrations of tetracycline in all tissues tested compared to the free tetracycline solution were observed. In conclusion, Tet-SLN can be prepared well by microemulsion method and subcutaneous injection of SLN provide a new perspective for drug sustained release.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 59 (2), 260-265, 2011

  公益社団法人 日本薬学会

キーワード

• tetracycline
• solid lipid nanoparticle
• microemulsion technique
• ternary phase diagram
• biodistribution

詳細情報

• CRID: 1390282679149811968
• NII論文ID: 130000405525
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.59.260
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 10951928
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10951928
• Web Site: http://www.jstage.jst.go.jp/article/cpb/59/2/59_2_260/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011335817
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可