Enzyme encapsulation in silica particles prepared using enzyme-assisted sol-gel reactions in ionic liquids
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- KATO Katsuya
- National Institute of Advanced Industrial Science and Technology (AIST)
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- NAKAGAKI Shun
- National Institute of Advanced Industrial Science and Technology (AIST) Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology
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- NISHIDA Masakazu
- National Institute of Advanced Industrial Science and Technology (AIST)
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- HIRAO Kiyoshi
- National Institute of Advanced Industrial Science and Technology (AIST) Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology
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Several studies have demonstrated the use of biomimetic approaches in the synthesis of a variety of inorganic materials. In this study, a low-temperature and natural pH procedure for the synthesis of silica was developed using hydrolytic enzymes. For the preparation of silica particles at room temperature and pH 7, a screening test for enzyme-catalyzing direct hydrolysis and polycondensation of tetraethoxysilane by using 5 types of hydrolases; pepsin, trypsin, esterase, and two lipases were chosen as biocatalysts; moreover, these enzymes can promote the precipitation of silica within 3 days. The 29Si nuclear magnetic resonance (NMR) measurements indicated that the enzyme activity in ionic liquids was higher than that in aqueous buffer solutions, and that the obtained silica particles were highly polycondensed. Under ionic liquid conditions, it was observed that enzymes were effectively encapsulated in the sol–gel silica material. In addition, for siloxane bond formation the catalytic activity of silica–enzyme composites was higher than that of free solution enzymes.
収録刊行物
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- Journal of the Ceramic Society of Japan
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Journal of the Ceramic Society of Japan 119 (1386), 140-143, 2011
公益社団法人 日本セラミックス協会
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詳細情報 詳細情報について
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- CRID
- 1390282680263078656
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- NII論文ID
- 130000420866
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- NII書誌ID
- AA12229489
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- ISSN
- 13486535
- 18820743
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- NDL書誌ID
- 11006596
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可