Inhibitory Effect of Bovine Lactoferrin on Human Parainfluenza Virus Type 2 Infection

• Yamamoto Hidetaka

  Department of Microbiology, Suzuka University of Medical Science

• Ura Yukari

  Department of Microbiology, Suzuka University of Medical Science

• Tanemura Miho

  Department of Microbiology, Suzuka University of Medical Science

• Koyama Aoi

  Department of Microbiology, Suzuka University of Medical Science

• Takano Sayaka

  Department of Microbiology, Suzuka University of Medical Science

• Uematsu Jun

  Department of Microbiology, Suzuka University of Medical Science

• Kawano Mitsuo

  Department of Microbiology, Mie University Graduate School of Medicine

• Tsurudome Masato

  Department of Microbiology, Mie University Graduate School of Medicine

• O'Brien Myles

  Graduate School of Mie Prefectural College of Nursing

• Komada Hiroshi

  Department of Microbiology, Graduate School of Clinical Nutrition, Suzuka University of Medical Science

抄録

Lactoferrin (Lf) is a multifunctional protein that has inhibitory activity against microorganisms. In this study, the effects of Lf on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK₂ cells were investigated. Lf inhibited cell fusion and hemadsorption induced by hPIV-2. However, virus RNA synthesis was only slightly inhibited by Lf. In addition, indirect immunofluorescence study showed that virus protein syntheses were not completely inhibited by Lf. Using a recombinant, green fluorescence protein-expressing hPIV-2 (rghPIV-2), it was found that virus entry into cells were considerably inhibited by Lf, but cell-to-cell spread was not inhibited. The number of viruses produced by the cells were determined, and it was found that Lf reduced the number of released viruses to about 1/300 compared with that of positive control. Lf bound to cell surface within 10 min at early phase of infection, it assembled gradually, and many aggregates were observed at 120 min. These results indicated that Lf considerably inhibited virus adsorption to the surface of the cells by binding to the cell surface and prevented hPIV-2 infection.

収録刊行物

• ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ

  ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ 56 (5), 613-617, 2010

  公益社団法人 日本薬学会

キーワード

• lactoferrin
• inhibition
• parainfluenza virus type 2

詳細情報

• CRID: 1390282679473801728
• NII論文ID: 130000425098
• NII書誌ID: AA11316464
• DOI: 10.1248/jhs.56.613
• COI: 1:CAS:528:DC%2BC3cXhtlags7vK
• ISSN: 13475207
• ISSN: 13449702
• NDL書誌ID: 10825254
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10825254
• Web Site: http://www.jstage.jst.go.jp/article/jhs/56/5/56_5_613/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011066470
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可