Inhibitory Effects of a Tryptamine Derivative on Ultraviolet Radiation-Induced Apoptosis in MC3T3-E1 Mouse Osteoblasts

DOI Web Site 被引用文献1件 参考文献28件
  • Mikami Yoshikazu
    Department of Anatomy, Nihon University School of Dentistry, Japan Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Japan
  • Senoo Motoki
    Department of Anatomy, Nihon University School of Dentistry, Japan
  • Lee Mio
    Department of Anatomy, Nihon University School of Dentistry, Japan
  • Yamada Kiyoshi
    Department of Microbiology, Nihon University School of Dentistry, Japan Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, Japan
  • Ochiai Kuniyasu
    Department of Microbiology, Nihon University School of Dentistry, Japan Division of Immunology and Pathobiology, Dental Research Center, Nihon University School of Dentistry, Japan
  • Honda Masaki J.
    Department of Anatomy, Nihon University School of Dentistry, Japan Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Japan
  • Watanabe Eri
    Laboratory of Diagnostic Medicine, The Institute of Medical Science, The University of Tokyo, Japan
  • Watanabe Nobukazu
    Laboratory of Diagnostic Medicine, The Institute of Medical Science, The University of Tokyo, Japan
  • Somei Masanori
    Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan
  • Takagi Minoru
    Department of Anatomy, Nihon University School of Dentistry, Japan Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Japan

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MS-IPA1 is a new synthetic compound that is synthesized from tryptamine. Recently, our group demonstrated that SST-VED-I-1, which has a similar chemical structure to MS-IPA1, inhibits starvation-induced apoptosis in osteoblasts. However, the effects of MS-IPA1 on apoptosis in osteoblasts have not yet been examined. Therefore, this study examined the effects of this compound on apoptosis in osteoblasts. In this study, MC3T3-E1 mouse osteoblasts were used and apoptosis was induced by ultraviolet radiation (UV). We investigated the effect of MS-IPA1 on apoptosis by analyzing caspase3/7 activity, translocation of phosphatidylserine (PS), and mRNA expression levels of Bcl-2 and Bax. In addition, it was investigated whether MS-IPA1 affects cell proliferation and cell cycle progression. We found that MS-IPA1 had no effect on cell proliferation or cell cycle progression. However, MS-IPA1 suppressed UV-induced cell death in a dose-dependent manner, which was accompanied with the inhibition of caspase activation and translocation of PS. Furthermore, after UV exposure, Bcl-2 expression was increased in the MS-IPA1–treated cells as compared to that in the vehicle-treated cells. In contrast, Bax expression was decreased in the MS-IPA1–treated cell as compared to that in the vehicle-treated cells. These results suggest that MS-IPA1 has an inhibitory effect on apoptosis in osteoblasts through a Bcl-2 family-dependent signaling pathway.

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