In Vivo Cell Tracking of Canine Allogenic Mesenchymal Stem Cells Administration via Renal Arterial Catheterization and Physiopathological Effects on the Kidney in Two Healthy Dogs

• YOO Jong-Hyun

  BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases and Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University

• PARK Chul

  Department of Internal Medicine, College of Veterinary Medicine, Chonbuk National University

• JUNG Dong-In

  Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Gyeongsang National University

• LIM Chae-Young

  BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases and Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University

• KANG Byeong-Teck

  BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases and Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University

• KIM Jung-Hyun

  BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases and Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University

• PARK Jung-Won

  National Veterinary Research & Quarantine Service

• KIM Jae-Hoon

  College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University

• PARK Hee-Myung

  BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases and Departments of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University

抄録

Stem cell therapy is being special premise for various renal diseases. However, there is limited literature on localization and pathologic and functional effects of allogenic mesenchymal stem cells (MSCs) in healthy dogs. Two healthy dogs were included in this study. Canine MSCs (cMSCs) were cultured from canine bone marrow and incubated with superparamagnetic iron oxide (SPIO) for in vivo cell tracking via MR imaging. The dogs were given the MSC (3 × 10⁶ cells) into a renal artery via femoral artery catheterization. Follow-up serial renal assessments included ultrasonography and MRI, serum chemistry, urine analysis, and renal clearance tests. The dogs were euthanized at days 8 and 35 respectively for histopathologic evaluation of kidney. Strong hypointensity in MRI was detected in the treated renal cortex the day after cMSCs infusion. However they disappeared from MR image by the 8th day. Of the serum chemistry tests, serum hepatic enzymes (ALT, AST) were significantly elevated for one week after cMSCs treatment. Histopathological findings also revealed infiltration of SPIO-containing cells into the parenchyma of kidney. On 35th day, histopathology, glomerular atrophy, tubular necrosis, and mineralization were found in the subcapsular cortex, with fibrosis of the interstitial tissues. In vivo MRI studies of stem cells were useful in determining the sequential location of stem cells in the renal parenchyma of healthy dogs. Allogenic stem cells administered via renal artery caused inflammation, tubular necrosis, mineralization, and fibrosis without functional complications.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 73 (2), 269-274, 2011

  公益社団法人 日本獣医学会

キーワード

• bone marrow
• canine
• kidney
• mesenchymal stem cells (MSCs)

詳細情報

• CRID: 1390282681406981760
• NII論文ID: 130000444193
• NII論文ID: 50007770128
• DOI: 10.1292/jvms.10-0044
• ISSN: 13477439
• ISSN: 09167250
• Web Site: http://www.jstage.jst.go.jp/article/jvms/73/2/73_10-0044/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011178275
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可