Pharmacology: Nitrergic inhibition of tachykininergic neuro-muscular transmission via cyclic GMP in the hamster ileum

• Matsuyama Hayato

  Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University

• UNNO Toshihiro

  Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University

• KOMORI Seiichi

  Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University

• Takewaki Tadashi

  Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University

書誌事項

タイトル別名

• Nitrergic Inhibition of Tachykininergie Neuro-Muscular Transmission via Cyclic GMP in the Hamster Ileum

抄録

The present study was designed to explore the inhibitory mechanism by nitric oxide (NO) of the tachykininergic neuro-muscular transmissions in the hamster ileum. In the presence of guanethidine (1 μM), atropine (0.5 μM), nifedipine (0.1 μM) and apamin (100 nM), electrical field stimuli (EFS; 0.5 ms duration, 15 V) evoked non-adrenergic, non-cholinergic excitatory junction potentials (EJPs) in circular smooth muscle cells. The EJPs were markedly inhibited by the tachykinin NK1 receptor antagonists [D-Pro⁴, D-Trp^7,9]-SP(4-11) (3 μM). Both the NO synthase inhibitor N^G-nitro-L-arginine methyl ester (L-NAME, 200 μM) and the soluble guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, 10 μM), did not affect on the resting membrane potentials, but enhanced the tachykininergic EJPs. In the presence of L-NAME (200 μM), exogenously applied NO (10 μM) and the membrane permeable analogue of guanosine 3',5'-cyclic monophosphate (cGMP), 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP, 3 mM), significantly inhibited the tachykininergic EJPs. Application of EFS (0.5 msec duration, 15 V) with trains of 20 pulses at 20 Hz increased amount of released substance P (SP). The release of SP was further increased by the treatment of L-NAME or ODQ, but markedly reduced by exogenously applied NO and 8-Br-cGMP. These results suggest that the endogenous NO may inhibit the tachykininergic neuro-muscular transmissions by the decrease of SP release from the tachykininergic neurons, possibly through a guanylate cyclase-cGMP-dependent mechanism in the hamster ileum.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 73 (4), 453-458, 2011

  公益社団法人 日本獣医学会

キーワード

• excitatory junction potential
• nitric oxide
• small intestine
• smooth muscle
• substance P

詳細情報

• CRID: 1390282681407918720
• NII論文ID: 130000444585
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.10-0425
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 11104748
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11104748
• Web Site: http://www.jstage.jst.go.jp/article/jvms/73/4/73_10-0425/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可