Differentiation of Stromal-Vascular Cells Isolated from Canine Adipose Tissues in Primary Culture.

• WU Peixing

  Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University

• SATO Kota

  Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University

• YUKAWA Sachiyo

  Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University

• HIKASA Yoshiaki

  Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University

• KAGOTA Katsumoto

  Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University

抄録

A culture condition supporting adipocyte differentiation of stromal-vascular (S-V) cells isolated from canine adipose tissues was established. Morphological observation and determination of glycerol-3-phosphate dehydrogenase (GPDH) activity were used as the criteria for adipocyte differentiation. After reaching confluence, the cells were able to undergo terminal adipocyte differentiation by treatment with 100 μM indomethacin, 10 μg/m l insulin and 0.5 mM 1-methyl-3-isobutylxanthine (MIX) in medium supplemented with 5% fetal calf serum (FCS). In the absence of either indomethacin or insulin, the S-V cells did not undergo adipose conversion and GPDH activity was not increased, indicating that both indomethacin and insulin play essential roles in this culture system. The S-V cells from inguinal adipose tissues exhibited the greatest increase in GPDH activity among the four depots (inguinal > abdominal-subcutaneous > perirenal > omental), demonstrating that adipocyte differentiation was also intensely dependent on anatomic sites from which the S-V cells were derived. Interestingly, dimethylsulfoxide (DMSO) was found to accelerate adipocyte differentiation in combination with indomethacin and insulin. Under this condition, up to 90% of the cells displayed adipocyte phenotypes and the GPDH activity reached 1288 ± 441 mU/mg protein. This culture system may be useful for investigating other adipogenic factors as well as anti-adipogenic factors involved in the regulation of canine adipose tissue development.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 63 (1), 17-23, 2001

  公益社団法人 日本獣医学会

キーワード

• adipocyte differentiation
• canine
• cell culture
• dimethylsulfoxide
• indomethacin

詳細情報

• CRID: 1390282681401171328
• NII論文ID: 130000449795
• NII論文ID: 110003920545
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.63.17
• COI: 1:CAS:528:DC%2BD3MXhtFCnt7k%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 5647151
• PubMed: 11217057
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5647151
• Web Site: http://www.jstage.jst.go.jp/article/jvms/63/1/63_1_17/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2001128236
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可