The lin genes for γ-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids

• TABATA Michiro

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• ENDO Ryo

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• ITO Michihiro

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• OHTSUBO Yoshiyuki

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• KUMAR Ashwani

  Environmental Biotechnology Section, Industrial Toxicology Research Center

• TSUDA Masataka

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

• NAGATA Yuji

  Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

書誌事項

タイトル別名

• The <I>lin</I> Genes for γ-Hexachlorocyclohexane Degradation in <I>Sphingomonas</I> sp. MM-1 Proved to Be Dispersed across Multiple Plasmids
• The lin genes for g hexachlorocyclohexane degradation in Sphingomonas sp MM 1 proved to be dispersed across multiple plasmids
• The lin genes for gamma-hexachlorocyclohexane degradation in Sphingomonas sp. MM-1 proved to be dispersed across multiple plasmids.

抄録

A γ-hexachlorocyclohexane (HCH)-degrading bacterium, Sphingomonas sp. MM-1, was isolated from soil contaminated with HCH isomers. Cultivation of MM-1 in the presence of γ-HCH led to the detection of five γ-HCH metabolites, γ-pentachlorocyclohexene, 2,5-dichloro-2,5-cyclohexadiene-1,4-diol, 2,5-dichlorohydroquinone, 1,2,4-trichlorobenzene, and 2,5-dichlorophenol, strongly suggesting that MM-1 has the lin genes for γ-HCH degradation originally identified in the well-studied γ-HCH-degrading strain Sphingobium japonicum UT26. Southern blot, PCR amplification, and sequencing analyses indicated that MM-1 has seven lin genes for the conversion of γ-HCH to β-ketoadipate (six structural genes, linA to linF, and one regulatory gene, linR). MM-1 carried four plasmids, of 200, 50, 40, and 30 kb. Southern blot analysis revealed that all seven lin genes were dispersed across three of the four plasmids, and that IS6100, often found close to the lin genes, was present on all four plasmids.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (3), 466-472, 2011

  公益社団法人 日本農芸化学会

キーワード

• γ-hexachlorocyclohexane
• <I>Sphingomonas</I>
• plasmid
• IS<I>6100</I>

詳細情報

• CRID: 1390001206477142400
• NII論文ID: 10028201533
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100652
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 11051551
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11051551
• Web Site: https://www.tandfonline.com/doi/pdf/10.1271/bbb.100652
• 本文言語コード: en
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