Protein Kinase B (Akt) Promotes Pathological Angiogenesis in Murine Model of Oxygen-Induced Retinopathy

• Wang Peng

  Department of Ophthalmology, Xiangya Hospital, Central South University

• Tian Xiao-Feng

  Department of Ophthalmology, Xiangya Hospital, Central South University

• Rong Jun-Bo

  Department of Ophthalmology, Xiangya Hospital, Central South University

• Liu Dan

  Department of Ophthalmology, Xiangya Hospital, Central South University

• Yi Guo-Guo

  Department of Ophthalmology, Xiangya Hospital, Central South University

• Tan Qian

  Department of Ophthalmology, Xiangya Hospital, Central South University

抄録

Akt, or protein kinase B, is an important signaling molecule that modulates many cellular processes such as cell growth, survival, and metabolism. However, the vivo roles and effectors of Akt in retinal angiogenesis are not explicitly clear. We therefore detected the expression of Akt using Western blotting or RT-PCR technologies in an animal model of oxygen-induced retinopathy, and investigated the effects of recombinant Akt on inhibiting vessels loss and Akt inhibitor on suppressing experimental retinal neovascularization in this model. We showed that in the hyperoxic phase of oxygen-induced retinopathy, the expression of Akt was greatly suppressed. In the hypoxic phase, the expression of Akt was increased dramatically. No significant differences were found in normoxic groups. Compared with control groups, administration of the recombinant Akt in the first phase of retinopathy markedly reduced capillary-free areas, while the administration of the Akt inhibitor in the second phase of retinopathy significantly decreased retinal neovascularization but capillary-free areas. These results indicate that Akt play a critical role in the pathological process (vessels loss and neovascularization) of mouse model of oxygen-induced retinopathy, which may provide a valubale therapeutic tool for ischemic-induced retinal diseases.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 44 (2), 103-111, 2011

  日本組織細胞化学会

キーワード

• recombinant Akt
• Akt inhibitor
• retinal vessel loss
• retinal neovascularization
• oxygen-induced retinopathy

詳細情報

• CRID: 1390001204863380096
• NII論文ID: 130000648762
• DOI: 10.1267/ahc.10038
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/44/2/44_10038/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012103593
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可