Characterization of Gingival Th1, Th2 and Th17 Cells in Murine Periodontitis Model

• Kono Tetsuro

  Nihon University Graduate School of Dentistry at Matsudo, Renascent Dentistry

• Kobayashi Ryoki

  Departments of Pediatric Dentistry and Microbiology, The Immunobiology Vaccine Center, The University of Alabama at Birmingham

• Fujihashi Kohtaro

  Departments of Pediatric Dentistry and Microbiology, The Immunobiology Vaccine Center, The University of Alabama at Birmingham

抄録

Cellular and molecular mechanisms of the immune system influencing oral bone metabolism remain to be elucidated. In this study, we characterize the mucosal cytokine production by CD4⁺ T cells in the inflamed gingiva of mice infected with Porphyromonas gingivalis (P. gingivalis). A murine periodontal disease model with alveolar bone loss showed significant levels of FimA-specific salivary secretory IgA and plasma IgG Ab responses. Further, IL-6 and TNF-α production was elevated when compared with sham-infected mice. The frequencies of Th1 (IFN-γ), Th2 (IL-4) and Th17 (IL-17) producing CD4⁺ T cells were also increased in P. gingivalis infected mice. Among these cytokines, a significantly higher frequency of IFN-γ producing CD4⁺ T cells was noted. The kinetics of intracellular cytokine analyses revealed a significantly increased frequency of IFN-γ-, IL-4- and IL-17-producing CD4⁺ T cells one day after infection. Further, the frequency of IFN-γ producing CD4⁺ T cells was maintained throughout the experimental period. Although IL-4 and IL-17 production was intact until 15 days after the infection, the numbers of CD4⁺ T cells producing these cytokines were reduced thereafter. These results indicate that Th1-type CD4⁺ T cells play distinct roles in the induction and regulation of periodontal disease when compared with Th2- and Th17-type cytokine-producing CD4⁺ T cells.

収録刊行物

• ＩＪＯＭＳ

  ＩＪＯＭＳ 9 (3), 213-219, 2011

  日本大学松戸歯学部 口腔科学研究所

キーワード

• CD4⁺ T cells
• periodontal disease
• cytokine
• gingival mucosa

詳細情報

• CRID: 1390282680189287936
• NII論文ID: 130000652912
• DOI: 10.5466/ijoms.9.213
• ISSN: 21854254
• ISSN: 13479733
• Web Site: http://www.jstage.jst.go.jp/article/ijoms/9/3/9_3_213/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012103033
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可