Intrathecal Disposition of ARTCEREB Irrigation and Perfusion Solution for Cerebrospinal Surgery in Rats

• Morioka Yujiro

  Research and Development Center, Otsuka Pharmaceutical Factory, Inc.

• Nishimura Masuhiro

  Research and Development Center, Otsuka Pharmaceutical Factory, Inc.

• Takehara Hiroaki

  Research and Development Center, Otsuka Pharmaceutical Factory, Inc.

• Doi Kazuhisa

  Research and Development Center, Otsuka Pharmaceutical Factory, Inc.

• Naito Shinsaku

  Research and Development Center, Otsuka Pharmaceutical Factory, Inc.

• Yamauchi Aiko

  Department of Pharmaceutical Information Science, Graduate School of Pharmaceutical Science, The University of Tokushima

抄録

We investigated the disposition of ARTCEREB_® irrigation and perfusion solution (Artcereb) during intrathecal perfusion in a lateral ventricle-cisternal perfusion model in conscious rats. In this perfusion model, the perfusion rate was set at 0.35 ml/kg/h, taking into consideration the clinical perfusion rate (500 ml/60 kg/d). The influence of Artcereb on electrolytes in cerebrospinal fluid (CSF) and blood were then investigated. After 24 h of ventriculocisternal perfusion with Artcereb using the push-pull method, output of K⁺, Na⁺ and Cl⁻ to the cistern magna was very similar to input of these electrolytes in Artcereb infused intraventricularly. Recovery rates of K⁺, Na⁺ and Cl⁻ after perfusion were 102%, 105% and 100% when calculated using the recovered perfusion solution. In addition, concentrations of K⁺, Na⁺ and Cl⁻ in blood remained almost constant at near baseline levels throughout perfusion. Thus, intrathecally perfused Artcereb did not affect electrolyte balance in the CSF and blood. To confirm the dynamics of Artcereb distribution, a whole body autoradiography study was performed at 1 and 6 h after perfusion with ¹⁴C-inulin-added Artcereb. Radioactivity was detected in the entire CSF space of the brain, and the cribriform plate in the nasal cavity, and the cerebrospinal cavity. Radioactivity was observed in the bladder, thus suggesting that some ¹⁴C-inulin was transferred to the bloodstream via a physiological route, and was excreted renally.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (5), 688-692, 2011

  公益社団法人 日本薬学会

キーワード

• intrathecal perfusion
• ARTCEREB_®
• artificial cerebrospinal fluid
• rat

詳細情報

• CRID: 1390282679601901952
• NII論文ID: 130000657781
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.688
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 11057909
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11057909
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/5/34_5_688/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012069545
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
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