Influence of Ascorbic acid (AsA) Concentration in Culture Medium on Mechanical Property of Regenerated Cartilage

• OMATA Seiji

  Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University

• SAWAE Yoshinori

  Department of Mechanical Engineering, Faculty of Engineering, Kyushu University

• MURAKAMI Teruo

  Department of Mechanical Engineering, Faculty of Engineering, Kyushu University

抄録

The chondrocyte-agarose construct has been employed as an experimental model in the cartilage tissue engineering context. We studied the influence of L-Ascorbic acid (AsA) concentration in culture medium on mechanical property of regenerated cartilage. Cylindrical chondrocyte-agarose constructs with a diameter of 4 mm and a height of 2.5 mm were prepared as test specimens. Chondrocytes isolated from metacarpal-phalangeal joint of steers were seeded in 1 g/mL agarose gel to give an initial cell density of 1×10⁷ cells/mL, and cultured in sterile culture medium within a humidified tissue culture incubator. We cultured by using levels of five AsA concentrations, 0.64, 2.2, 3.2 and 6.4 pmol/10⁹cells and without AsA. Culture medium was exchanged every two days. After culture periods of 1, 8, 15 and 22 days, tangent modulus of the cultured constructs and glycosaminoglycan (GAG) biosynthesis were evaluated by the unconfined compression test and the dimethylmethylene blue (DMMB) assay, respectively. The structural organization of the elaborated tissue was also examined morphologically by the confocal laser scanning microscope (CLSM). Results indicated that the tangent modulus of the cultured constructs increased with increasing the AsA concentration in culture medium. In addition, the growth rate of the tangent modulus was proportional to the AsA concentration. The increased amount of AsA would contribute to the accelerated self-assembly of the collagen fiber network and resultant improvement in the mechanical property, since the reduction ability of AsA could enhance the procollagen expression in cultured chondrocytes.

収録刊行物

• Journal of Environment and Engineering

  Journal of Environment and Engineering 6 (2), 416-425, 2011

  一般社団法人 日本機械学会

キーワード

• Regenerated cartilage
• Chondrocyte
• Ascorbic acid
• Agarose gel
• Tangent modulus
• Type II collagen
• Glycosaminoglycan

詳細情報

• CRID: 1390001205261334656
• NII論文ID: 130000667012
• DOI: 10.1299/jee.6.416
• ISSN: 1880988X
• Web Site: http://www.jstage.jst.go.jp/article/jee/6/2/6_2_416/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可