Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry
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- Okazawa Atsushi
- Graduate School of Engineering, Osaka University
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- Hori Katsuhito
- Graduate School of Engineering, Osaka University
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- Okumura Ryohei
- Graduate School of Engineering, Osaka University
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- Izumi Yoshihiro
- Graduate School of Engineering, Osaka University
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- Hata Naoki
- Graduate School of Engineering, Osaka University
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- Bamba Takeshi
- Graduate School of Engineering, Osaka University
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- Fukusaki Eiichiro
- Graduate School of Engineering, Osaka University
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- Ono Eiichiro
- R&D Planning Division, Suntory Holdings, Ltd.
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- Satake Honoo
- Suntory Insitutute of Bioorganic Research
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- Kobayashi Akio
- Graduate School of Engineering, Osaka University
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抄録
Lignans are phenylpropanoid dimers in which the monomers are linked by the central carbon (C8) atoms. Because many lignans have physiological effects, including antioxidant activity, they are now in demand as components in health foods. However, the lignan biosynthetic pathways in plants are only now being understood. Recently, lariciresinol was detected in Arabidopsis thaliana. This observation indicated the existence of common lignan biosynthetic pathways in A. thaliana, despite a low amount of lignans other than lariciresinol glycosides. In this study, we established a highly sensitive analytical method that enables quantification of both glycoside and aglycone forms of lignans in A. thaliana simultaneously using capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry. Some lignans not previously detected in A. thaliana were quantified in extracts of both roots and shoots. Our method can be used for the comprehensive analysis of lignans in small samples from mutants and transformants. This method will be utilized to elucidate the metabolic pathways and physiological roles of lignans as well as the regulation of their biosynthesis in plants.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 28 (3), 287-293, 2011
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679306299136
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- NII論文ID
- 10029399367
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC3MXpvVWru7Y%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 11133766
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可