Tissue culture note: Adventitious bud formation through nodule induction by thidiazuron in cultured leaf segments of the Japanese persimmon (Diospyros kaki Thunb.)
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- Yokoyama Tomosaburo
- Graduate School of Science and Engineering, Saitama University
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- Moriyasu Yuji
- Graduate School of Science and Engineering, Saitama University
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- Sugawara Yasutake
- Graduate School of Science and Engineering, Saitama University
書誌事項
- タイトル別名
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- Adventitious bud formation through nodule induction by thidiazuron in cultured leaf segments of the Japanese persimmon (Diospyros kaki Thunb.)
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Multiple meristematic nodules were induced on leaf segments from the Japanese persimmon (Diospyros kaki Thunb.) ‘Fuyu’ that were cultured on a solidified half-strength Murashige and Skoog's medium containing 2% (w/v) sucrose and 1 μM thidiazuron (TDZ). The nodules 1 to 3 mm in diameter were formed in the cut ends and abaxial side of the leaf segments two weeks after inoculation. The nodule changed from pale green to dense green and multiplied to form many daughter nodules. Adventitious buds were thereafter formed on the nodules, suggesting that the meristematic nodule is an intermediate structure for bud differentiation. The adventitious buds grew poorly on the medium with TDZ, but resumed growth and developed into shoots after transfer to the medium containing 2% (w/v) sucrose and 10 μM zeatin. The meristematic nodules survived more than one year on the medium containing 2% (w/v) sucrose and 1 μM TDZ without transfer, and did not lose their ability to form adventitious buds for more than 5 years when transferred regularly to a fresh medium. These results suggest that the meristematic nodule is a promising material for propagation and long-term conservation of this plant.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 28 (3), 339-344, 2011
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679306288768
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- NII論文ID
- 10029399554
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BC3MXpvVWqsrg%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 11133900
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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