HPLC Determination of Strychnine and Brucine in Rat Tissues and the Distribution Study of Processed Semen Strychni

• CHEN Jun

  College of Pharmacy, Nanjing University of Chinese Medicine

• HOU Ting

  College of Pharmacy, Nanjing University of Chinese Medicine

• FANG Yun

  Affiliated Drum Tower Hospital of Nanjing University Medical School

• CHEN Zhi-peng

  College of Pharmacy, Nanjing University of Chinese Medicine

• LIU Xiao

  Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine

• CAI Hao

  Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine

• LU Tu-lin

  College of Pharmacy, Nanjing University of Chinese Medicine

• YAN Guo-jun

  College of Pharmacy, Nanjing University of Chinese Medicine

• CAI Bao-chang

  Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine

Abstract

  A simple and low-cost HPLC method with UV absorbance detection was developed and validated to simultaneously determine strychnine and brucine, the most abundant alkaloids in the processed Semen Strychni, in rat tissues (kidney, liver, spleen, lung, heart, stomach, small intestine, brain and plasma). The tissue samples were treated with a simple liquid-liquid extraction prior to HPLC. The LOQs were in the range of 0.039∼0.050 μg/ml for different tissue or plasma samples. The extraction recoveries varied from 71.63 to 98.79%. The linear range was 0.05-2 μg/ml with correlation coefficient of over 0.991. The intra- and inter-day precision was less than 15%. Then the method was used to measure the tissue distribution of strychnine and brucine after intravenous administration of 1 mg/kg crude alkaloids fraction (CAF) extracted from the processed Semen Strychni. The results revealed that strychnine and brucine possessed similar tissue distribution characterization. The highest level was observed in kidney, while the lowest level was found in brain. It was indicated that kidney might be the primary excretion organ of prototype strychnine and brucine. It was also deduced that strychnine and brucine had difficulty in crossing the blood-brain barrier. Furthermore, no long-term accumulation of strychnine and brucine was found in rat tissues.<br>

Journal

• YAKUGAKU ZASSHI

  YAKUGAKU ZASSHI 131 (5), 721-729, 2011-05-01

  The Pharmaceutical Society of Japan

Keywords

• Semen Strychni
• HPLC
• tissue distribution
• strychnine
• brucine

Details

• CRID: 1390282681103495424
• NII Article ID: 130000678854
• NII Book ID: AN00284903
• DOI: 10.1248/yakushi.131.721
• ISSN: 13475231
• ISSN: 00316903
• NDL BIB ID: 11112127
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11112127
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/131/5/131_5_721/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012013401
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed